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分子网络以及基于生物测定法的从罗斯属植物中制备和分离活性提取物及成分的研究

Molecular Networking and Bioassay-Guided Preparation and Separation of Active Extract and Constituents from Roth.

作者信息

Le Duc Dat, Yu Soojung, Dang Thinhulinh, Lee Mina

机构信息

College of Pharmacy, Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Jeonnam, Republic of Korea.

出版信息

Antioxidants (Basel). 2023 Oct 18;12(10):1876. doi: 10.3390/antiox12101876.

DOI:10.3390/antiox12101876
PMID:37891955
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10604256/
Abstract

Molecular networking drove the selection of material from organs that targeted active flavonoid glycosides. To optimize the extraction process, the flowers of were used to produce an anti-inflammatory extract. The effects of variables-organic solvent ratio; extraction time; and temperature-were investigated by the response of anti-inflammatory activity. Bioactivities-guided experiments helped identify fractions with high total phenolic and flavonoid content as well as antioxidant potential. Furthermore, one new compound (), 19 first isolated together, and two known compounds were obtained and identified from the active fraction of this plant. Among them, compounds ( and ) were first reported for nuclear magnetic resonance (NMR) data from this study. All the isolates were evaluated for their anti-inflammatory capacity throughout, modulating nitric oxide (NO), interleukin (IL)-1β, and IL-8 production. Active compounds were further investigated for their regulation and binding affinity to the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins by Western blot and in silico approaches, respectively. The findings of this study suggested that the developed extract method, active fraction, and pure components should be further investigated as promising candidates for treating inflammation and oxidation.

摘要

分子网络驱动了从靶向活性黄酮苷的器官中选择材料。为了优化提取工艺，使用[植物名称]的花来制备抗炎提取物。通过抗炎活性的响应研究了有机溶剂比例、提取时间和温度等变量的影响。生物活性导向实验有助于鉴定出总酚和黄酮含量高以及具有抗氧化潜力的馏分。此外，从该植物的活性馏分中获得并鉴定了一种新化合物（[化合物名称]）、19种首次共同分离得到的化合物以及两种已知化合物。其中，化合物（[化合物编号]和[化合物编号]）的核磁共振（NMR）数据首次在本研究中报道。对所有分离物的抗炎能力进行了全面评估，检测其对一氧化氮（NO）、白细胞介素（IL）-1β和IL-8产生的调节作用。通过蛋白质印迹法和计算机模拟方法分别进一步研究了活性化合物对诱导型一氧化氮合酶（iNOS）和环氧合酶-2（COX-2）蛋白的调节作用和结合亲和力。本研究结果表明，所开发的提取方法、活性馏分和纯成分作为治疗炎症和氧化的有前景的候选物应进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/1161afabe75a/antioxidants-12-01876-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/5a0b4c430cac/antioxidants-12-01876-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/11fdf27aa2bc/antioxidants-12-01876-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/7b836dd2d27d/antioxidants-12-01876-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/04464556c780/antioxidants-12-01876-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/765799781713/antioxidants-12-01876-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/65c254e3c4e6/antioxidants-12-01876-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/f5058016ec2e/antioxidants-12-01876-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/9b564bbb8572/antioxidants-12-01876-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/1161afabe75a/antioxidants-12-01876-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/5a0b4c430cac/antioxidants-12-01876-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/11fdf27aa2bc/antioxidants-12-01876-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/7b836dd2d27d/antioxidants-12-01876-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/04464556c780/antioxidants-12-01876-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/765799781713/antioxidants-12-01876-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/65c254e3c4e6/antioxidants-12-01876-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/f5058016ec2e/antioxidants-12-01876-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/9b564bbb8572/antioxidants-12-01876-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c093/10604256/1161afabe75a/antioxidants-12-01876-g009.jpg

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