Shenzhen Clinical Research Center for Digestive Disease, Integrative Microecology Clinical Center, Shenzhen Key Laboratory of Gastrointestinal Microbiota and Disease, Shenzhen Technology Research Center of Gut Microbiota Transplantation, Shenzhen Hospital, Southern Medical University, Shenzhen 518100, China.
State Key Laboratory of Organ Failure Research, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510080, China.
Nutrients. 2023 Oct 16;15(20):4378. doi: 10.3390/nu15204378.
Ulcerative colitis (UC) is a chronic and recurrent inflammatory disease with an unknown pathogenesis and increasing incidence. The objective of this study is to investigate the impact of prophylactic treatment with on UC. The findings demonstrate that prophylactic supplementation of powder effectively mitigates disease symptoms in DSS-injured mice, while also reducing the secretion of pro-inflammatory cytokines. Furthermore, powder enhances the integrity of the intestinal mucosal barrier by up-regulating MUC2 protein expression and improving tight junction proteins (ZO-1, occludin, and claudin 1) in DSS-injured mice. Multiomics integration analyses revealed that powder not only reshaped gut microbiota composition, with an increase in , , and , but also exerted regulatory effects on various metabolic pathways including amino acid, glyoxylates, dicarboxylates, glycerophospholipids, and arachidonic acid. Subsequent analysis further elucidated the intricate interplay of gut microbiota, the intestinal mucosal barrier, and metabolites, suggesting that the microbiota-metabolite axis may involve the effect of on intestinal mucosal barrier repair in UC. Moreover, in vitro experiments demonstrated that peptides and polysaccharides, derived from , exerted an ability to change the gut microbiota structure of UC patients' feces, particularly by promoting the growth of . These findings suggest that regulatory properties of on gut microbiota may underlie the potential mechanism responsible for the protective effect of in UC. Consequently, our study will provide support for the utilization of as a whole food-based ingredient against the occurrence and development of UC.
溃疡性结肠炎（UC）是一种慢性、复发性炎症性疾病，其发病机制尚不清楚，发病率呈上升趋势。本研究旨在探讨预防性使用[药物名称]对 UC 的影响。研究结果表明，预防性补充[药物名称]粉末可有效减轻 DSS 损伤小鼠的疾病症状，同时减少促炎细胞因子的分泌。此外，[药物名称]粉末通过上调 MUC2 蛋白表达和改善 DSS 损伤小鼠的紧密连接蛋白（ZO-1、occludin 和 claudin 1）来增强肠道黏膜屏障的完整性。多组学整合分析表明，[药物名称]粉末不仅重塑了肠道微生物群落组成，增加了[细菌名称]、[细菌名称]、[细菌名称]和[细菌名称]，而且对包括氨基酸、乙醛酸、二羧酸、甘油磷脂和花生四烯酸在内的多种代谢途径发挥了调节作用。后续分析进一步阐明了肠道微生物群、肠道黏膜屏障和代谢物之间的复杂相互作用，表明微生物群-代谢物轴可能涉及[药物名称]对 UC 肠道黏膜屏障修复的作用。此外，体外实验表明，[药物名称]的肽和多糖能够改变 UC 患者粪便中的肠道微生物群落结构，特别是通过促进[细菌名称]的生长。这些发现表明，[药物名称]对肠道微生物群的调节特性可能是其在 UC 中发挥保护作用的潜在机制。因此，我们的研究将为将[药物名称]作为一种全食物成分用于预防和治疗 UC 提供支持。
