Department of Physiology, Key Laboratory of Physiology of Shanxi Province, Key Laboratory of Cellular Physiology of Ministry of Education, Shanxi Medical University, Taiyuan 030001, China.
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Nutrients. 2023 Jul 6;15(13):3054. doi: 10.3390/nu15133054.
Ulcerative colitis (UC) is evolving into a global burden with a substantially increasing incidence in developing countries. It is characterized by inflammation confined to mucosa and is recognized as an intestinal barrier disease. The intestinal microbiota plays a crucial role in UC pathogenesis. has long been appreciated as a healthy food and supplement worldwide and polysaccharides account for 60%. Here, we examined the amelioration of polysaccharides against acute colitis in mice induced by DSS and assessed the mediating role of gut microbiota. An integrated analysis of microbiome, metabolomics, and transcriptomics fully elaborated it markedly enhanced intestinal mucosal barrier function, including: increasing the relative abundance of , and ; decreasing microbiota-derived phosphatidylcholines and thromboxane 2 levels mapped to arachidonic acid metabolism; improving mucin2 biosynthesis and secretion; enhancing ZO-1 and occludin expression; reducing neutrophil infiltration; regulating the level of colitis-related inflammatory cytokines; involving inflammation and immune function-associated signaling pathways. Further, the mediation effect of gut microbiota was evaluated by administering a cocktail of antibiotics. In conclusion, our results demonstrated that polysaccharides predominantly reinforced the gut microbiota-mediated intestinal mucosal barrier to confer protection against UC and exhibited dramatic prebiotic-like functions, providing an alternative or complementary treatment for UC.
溃疡性结肠炎（UC）正在成为一个全球性的负担，在发展中国家的发病率显著增加。它的特点是炎症局限于粘膜，被认为是一种肠道屏障疾病。肠道微生物群在 UC 的发病机制中起着至关重要的作用。 长期以来一直被认为是一种健康的食物和补品，在全世界范围内，多糖占 60%。在这里，我们研究了 多糖对 DSS 诱导的急性结肠炎小鼠的改善作用，并评估了肠道微生物群的介导作用。微生物组、代谢组学和转录组学的综合分析充分阐述了它显著增强了肠道粘膜屏障功能，包括：增加 的相对丰度；减少微生物衍生的磷脂酰胆碱和血栓素 2 水平，映射到花生四烯酸代谢；改善粘蛋白 2 的生物合成和分泌；增强 ZO-1 和 occludin 的表达；减少中性粒细胞浸润；调节结肠炎相关炎症细胞因子的水平；涉及炎症和免疫功能相关的信号通路。此外，通过给予抗生素混合物来评估肠道微生物群的介导作用。总之，我们的结果表明， 多糖主要通过增强肠道微生物群介导的肠道粘膜屏障来预防 UC，并表现出明显的类似益生元的功能，为 UC 提供了一种替代或补充治疗方法。
