Postgraduate Program in Periodontology, Department of Periodontology, University of Indonesia, Jakarta 10430, Indonesia.
Department of Periodontology, Faculty of Dentistry, University of Indonesia, Jakarta 10430, Indonesia.
Medicina (Kaunas). 2023 Oct 6;59(10):1778. doi: 10.3390/medicina59101778.
: Periodontitis is an inflammatory disease in the supporting tissues of the teeth caused by specific microorganisms or groups of microorganisms. bacterium is the keystone pathogen in periodontitis, so even at low concentrations, it has a considerable influence on the oral community. Antimicrobials and antiplaque agents can be used as adjunctive therapy for periodontitis treatment. Konjac glucomannan (KGM), as a natural polysaccharide, has flavonoid (3,5-diacetyltambulin) and triterpenoids (ambylon) compounds that show antibacterial activity. This research aims to analyze the antibacterial activity of KGM on animal and in vitro periodontitis models. : The animal study divided 48 mice into four groups (control, KGM, periodontitis, KGM + periodontitis). Mice were given an intervention substance by oral gavage from day 1 to day 14, periodontitis was induced on day 7, and decapitation was performed on day 14. Samples from the right maxillary jaw of mice were used for histological preparations and morphometrics analysis. In vitro studies were carried out by adding several concentrations of KGM (25, 50, and 100 μg/mL) into a planktonic and biofilm. : In the animal model, KGM could prevent alveolar bone loss in the periodontitis mice model, both in histologic and morphometrics assessments. In vitro, KGM had antibacterial activity against with better bacteriostatic (15-23%) than bactericidal (11-20%) ability, proven by its ability to inhibit proliferation. : KGM can be considered to have the potential as an antibacterial agent to prevent periodontitis. The prevention of periodontitis may improve patient well-being and human quality of life.
牙周炎是一种由特定微生物或微生物群引起的牙齿支持组织的炎症性疾病。细菌是牙周炎的关键病原体，因此即使浓度较低，它对口腔群落也有相当大的影响。抗菌剂和抗菌斑剂可用作牙周炎治疗的辅助疗法。魔芋葡甘聚糖（KGM）作为一种天然多糖，具有黄酮类（3,5-二乙酰曲菌素）和三萜类（安比隆）化合物，具有抗菌活性。本研究旨在分析 KGM 对动物和体外牙周炎模型的抗菌活性。
动物研究将 48 只小鼠分为四组（对照组、KGM 组、牙周炎组、KGM+牙周炎组）。从第 1 天到第 14 天，小鼠通过口服灌胃给予干预物质，第 7 天诱导牙周炎，第 14 天断头。使用来自小鼠右上颌颌骨的样本进行组织学制备和形态计量学分析。体外研究通过向浮游生物和生物膜中添加几种浓度的 KGM（25、50 和 100μg/ml）来进行。
在动物模型中，KGM 可以预防牙周炎小鼠模型中的牙槽骨丢失，在组织学和形态计量学评估中均如此。在体外，KGM 对具有抗菌活性，其抑菌（15-23%）能力优于杀菌（11-20%）能力，这证明了其抑制增殖的能力。
KGM 可以被认为具有作为预防牙周炎的抗菌剂的潜力。预防牙周炎可以改善患者的幸福感和人类的生活质量。
