Department of Laboratory Medicine, Kaunas Klinikos, The Hospital of Lithuanian University of Health Sciences, 50161 Kaunas, Lithuania.
Department of Production Engineering, Kaunas University of Technology, 51424 Kaunas, Lithuania.
Medicina (Kaunas). 2023 Oct 12;59(10):1819. doi: 10.3390/medicina59101819.
Growing antibiotic resistance among bacteria is a global issue that is becoming harder and more expensive to solve. Traditional treatment options are becoming less effective, causing more fatal outcomes of nosocomial infections. Since the development of new antibiotics has stagnated in the last decade, a novel approach is needed. Graphene-based materials are being developed and tested for various applications, and the medical field is no exception. We tested 98 clinical strains for antibiotic resistance, AMP-C production and the effectiveness of a graphene oxide and silver nanoparticle hybrid nanocomposite. The disc diffusion method was used to determine antibiotic susceptibility results. Antibiotic discs containing cefotaxime, cloxacillin and clavulanate were used to detect AMP-C production. The effectiveness of the GO-Ag hybrid nanocomposite was determined by counting colony forming units (CFUs) after a suspension of and the GO-Ag hybrid nanocomposite was plated on MH agar and incubated overnight to grow colonies. : In our research, we found that strains are resistant to the majority of commonly used antibiotics. Antibiotic resistance levels and AMP-C production can be factors, indicating the better effectiveness of the graphene oxide and silver nanoparticle hybrid nanocomposite. : In this study, a GO-Ag hybrid nanocomposite was shown to have the potential to fight even the most problematic bacteria like .
细菌对抗生素的耐药性日益增强，这是一个全球性问题，且解决难度越来越大，成本也越来越高。传统的治疗选择效果越来越差，导致医院感染的致死率更高。由于过去十年新抗生素的研发陷入停滞，因此需要一种新的方法。
基于石墨烯的材料正在被开发并测试用于各种应用，医学领域也不例外。我们测试了 98 株临床菌株的抗生素耐药性、AMP-C 的产生以及氧化石墨烯和银纳米粒子杂化纳米复合材料的有效性。采用圆盘扩散法来确定抗生素敏感性结果。使用含有头孢噻肟、氯唑西林和克拉维酸的抗生素圆盘来检测 AMP-C 的产生。通过将 和氧化石墨烯-银杂化纳米复合材料的悬浮液涂在 MH 琼脂上并孵育过夜以生长菌落，来确定 GO-Ag 杂化纳米复合材料的有效性。结果表明：在我们的研究中，我们发现有 株菌株对大多数常用抗生素具有耐药性。抗生素耐药水平和 AMP-C 的产生可能是一个因素，这表明氧化石墨烯和银纳米粒子杂化纳米复合材料的效果更好。结论：在这项研究中，证明了 GO-Ag 杂化纳米复合材料具有对抗最棘手细菌（如 ）的潜力。
