青霉素筛选大肠杆菌脱氧核糖核酸修复突变体
Penicillin selection of Escherichia coli deoxyribonucleic acid repair mutants.
作者信息
Sancar A, Rupert C S
出版信息
J Bacteriol. 1979 Jun;138(3):779-82. doi: 10.1128/jb.138.3.779-782.1979.
Abstract
A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.
摘要
已开发出一种快速方法来分离大肠杆菌的紫外线敏感突变体，即通过用低通量远紫外线辐射诱导修复缺陷细胞的生长和/或分裂延迟，并用青霉素杀死仍继续生长和分裂的修复 proficient 细胞。利用这种技术，我们已实现对光复活能力较低（phr）细胞约 3000 倍的富集，并分离和鉴定了三个 phr 突变体。
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