von Wright A, Bridges B A
J Bacteriol. 1981 Apr;146(1):18-23. doi: 10.1128/jb.146.1.18-23.1981.
Mutations at the gyrB locus were found to decrease the degree of supercoiling of the Escherichia coli chromosome. The effect of a gyrB mutation on the repair of ultraviolet-induced deoxyribonucleic acid damage was studied by following the killing of strains of E. coli K-12 proficient and deficient in deoxyribonucleic acid repair. The effectiveness of both excision and postreplication types of deoxyribonucleic acid repair was found to be altered by this mutation, the former being apparently enhanced and the latter impaired.
研究发现,gyrB位点的突变会降低大肠杆菌染色体的超螺旋程度。通过追踪DNA修复能力正常和缺陷的大肠杆菌K-12菌株的存活率,研究了gyrB突变对紫外线诱导的DNA损伤修复的影响。结果发现,这种突变改变了切除修复和复制后修复两种类型的DNA修复的有效性,前者明显增强,后者受损。