Li Peipei, Hong Jinni, Wu Mingrou, Yuan Zhanhong, Li Dinghao, Wu Zhongdao, Sun Xi, Lin Datao
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-Sen University, Guangzhou 510080, China.
Microorganisms. 2023 Sep 28;11(10):2419. doi: 10.3390/microorganisms11102419.
snails play a crucial role in the transmission of the human blood fluke . The gut microbiota of intermediate hosts is known to influence their physiological functions, but little is known about its composition and role in snails. To gain insights into the biological characteristics of these freshwater intermediate hosts, we conducted metagenomic sequencing on and to investigate variations in their gut microbiota. This study revealed that the dominant members of the gut microbiota in belong to the phyla Bacteroidetes and Proteobacteria, which were also found to be the top two most abundant gut bacteria in . We identified Firmicutes, and as distinctive gut microbes in , while , and were found to be dependent features of the gut microbiota. We observed significant differences in the community structures and bacterial functions of the gut microbiota between the two host species. Notably, we found a distinctive richness of antibiotic resistance genes (ARGs) associated with various classes of antibiotics, including bacitracin, chloramphenicol, tetracycline, sulfonamide, penicillin, cephalosporin_ii and cephalosporin_i, fluoroquinolone, aminoglycoside, beta-lactam, multidrug and trimethoprim, in the digestive tracts of the snails. Furthermore, this study revealed the potential correlations between snail gut microbiota and the infection rate of using Spearman correlation analysis. Through metagenomic analysis, our study provided new insights into the gut microbiota of snails and how it is influenced by host species, thereby enhancing our understanding of variant patterns of gut microbial communities in intermediate hosts. Our findings may contribute to future studies on gastropod-microbe interactions and may provide valuable knowledge for developing snail control strategies to combat schistosomiasis in the future.
蜗牛在人类血吸虫的传播中起着至关重要的作用。已知中间宿主的肠道微生物群会影响它们的生理功能，但对于其在蜗牛体内的组成和作用却知之甚少。为了深入了解这些淡水中间宿主的生物学特性，我们对[具体品种1]和[具体品种2]进行了宏基因组测序，以研究它们肠道微生物群的变化。这项研究表明，[具体品种1]肠道微生物群的主要成员属于拟杆菌门和变形菌门，这两个门的细菌也是在[具体品种2]中含量最丰富的前两类肠道细菌。我们确定厚壁菌门、[具体菌属1]和[具体菌属2]是[具体品种1]中独特的肠道微生物，而[具体菌属3]、[具体菌属4]和[具体菌属5]是[具体品种2]肠道微生物群的依赖特征。我们观察到这两种宿主物种的肠道微生物群在群落结构和细菌功能上存在显著差异。值得注意的是，我们在蜗牛的消化道中发现了与各类抗生素（包括杆菌肽、氯霉素、四环素、磺胺类、青霉素、头孢菌素II和头孢菌素I、氟喹诺酮类、氨基糖苷类、β-内酰胺类、多药耐药类和甲氧苄啶）相关的独特丰富的抗生素抗性基因（ARGs）。此外，这项研究通过Spearman相关性分析揭示了蜗牛肠道微生物群与[血吸虫名称]感染率之间的潜在相关性。通过宏基因组分析，我们的研究为[蜗牛品种]的肠道微生物群以及宿主物种如何影响它提供了新的见解，从而加深了我们对中间宿主肠道微生物群落变异模式的理解。我们的发现可能有助于未来关于腹足动物与微生物相互作用的研究，并可能为未来制定控制蜗牛以对抗血吸虫病的策略提供有价值的知识。
