Di Teng, Li Yongping, Du Guangzu, He Yanyan, Wang Wenqian, Shen Yunfeng, Meng Jizhi, Xiao Wenxiang, Xiao Guanli, Chen Bin
State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
School of Agriculture, Yunnan University, Kunming 650500, China.
Microorganisms. 2023 Oct 8;11(10):2514. doi: 10.3390/microorganisms11102514.
(J.E.Smith) (Lepidoptera: Noctuidae) was first found in 2019 in Yunnan, China, and it was characterized as a corn strain; it was also found on rice strains there, and it damages rice in China, but little is known about the effect of host plant transfer on the intestinal microbiota and the activities of detoxification enzymes in the C-strain (corn strain) . The intestinal microbiota and the protective enzyme activity of that were transferred from rice plants were assessed, and the fourth generation of insects transferred from corn were studied; the gene types of that were transferred from rice plants were tested using mitochondrial gene sequences. The results showed that the intestinal microbiota in the C-strain were changed after the host transference, and the diversity and richness of the intestinal bacterial communities of the feeding on rice were significantly reduced after the transfer of the host from corn. The predominant species of intestinal bacteria of the on rice transferred from corn were and , with relative abundances of 28.7% and 66.68%; the predominant species of intestinal bacteria of the that were transferred from rice and feeding on corn were (22.35%) and (73.92%); and the predominant species of intestinal bacteria of feeding on corn was , with a relative abundance of 61.26%. The CAT (catalase) activity of the transferred from corn onto rice from corn was reduced, the POD (peroxidase) activity was significantly increased after the transfer from corn, and no significant variations were found for the SOD (superoxide dismutase), CarE (carboxylesterase), and GST (glutathione S-transferase) activities of after the host plant transfer. The results showed that after feeding on rice, the activities of CAT and POD in the in body changed in order to resist plant secondary metabolites from corn or rice, but there was no significant change in the detoxification enzymes in the body. In summary, switching the host plant between corn and rice induced variations in the intestinal microbiota in C-strain owing to the strain difference between the C-strain and the R-strain (rice strain), and this was consistent with the results of the activities of detoxification enzymes. The results indicat that changes in intestinal microbiota and physiological enzymes may be important reasons for the adaptive capacity of C-strain to rice.
（J.E.史密斯）（鳞翅目：夜蛾科）于2019年首次在中国云南被发现，其被鉴定为玉米品系；在当地的水稻品系上也有发现，它在中国会危害水稻，但关于寄主植物转移对C品系（玉米品系）肠道微生物群和解毒酶活性的影响知之甚少。评估了从水稻植株转移过来的该昆虫的肠道微生物群和保护酶活性，并研究了从玉米转移过来的第四代昆虫；使用线粒体基因序列检测了从水稻植株转移过来的该昆虫的基因类型。结果表明，C品系昆虫在寄主转移后肠道微生物群发生了变化，寄主从玉米转移到水稻后，取食水稻的该昆虫肠道细菌群落的多样性和丰富度显著降低。从玉米转移到水稻上的该昆虫肠道细菌优势种为和，相对丰度分别为28.7%和66.68%；从水稻转移过来并取食玉米的该昆虫肠道细菌优势种为（22.35%）和（73.92%）；取食玉米的该昆虫肠道细菌优势种为，相对丰度为61.26%。从玉米转移到水稻上的该昆虫的CAT（过氧化氢酶）活性降低，从玉米转移后POD（过氧化物酶）活性显著增加，寄主植物转移后该昆虫的SOD（超氧化物歧化酶）、CarE（羧酸酯酶）和GST（谷胱甘肽S -转移酶）活性未发现显著变化。结果表明，取食水稻后，该昆虫体内的CAT和POD活性发生变化以抵抗来自玉米或水稻的植物次生代谢产物，但其体内解毒酶无显著变化。综上所述，由于C品系和R品系（水稻品系）之间的品系差异，在玉米和水稻之间切换寄主植物会导致C品系昆虫肠道微生物群发生变化，这与解毒酶活性的结果一致。结果表明肠道微生物群和生理酶的变化可能是C品系昆虫对水稻适应能力的重要原因。
