Helander H F, Leth R, Olbe L
Anat Rec. 1986 Nov;216(3):373-80. doi: 10.1002/ar.1092160306.
Quantitative morphological data on normal human oxyntic mucosa were obtained from endoscopic biopsies in ten healthy male volunteers. Corpus mucosa was biopsied in the resting state and during maximal acid secretion and then processed for light and electron microscopy. Stereological analyses were carried out on sections comprising the entire thickness of the epithelial layer. About one-third of the mucosal volume was taken up by lamina propria and 15% by parietal cells. Counts of cells that displayed their nucleus in the sections revealed that in average of 12% of the epithelial cells were parietal cells, 43% were mucous cells, 40% were zymogen cells, and 4% were endocrine cells. Parietal cells displaying two nuclei were twice as large as those with only one nucleus. Six percent of the parietal cell volume was taken up by the nucleus, and 33% of the cytoplasmic volume was occupied by mitochondria. Stimulation of acid secretion resulted in a 76% increase in the secretory surface density; simultaneously there was a slight decrease in the mean size of the parietal cells and an increase in the relative volume of the nucleus. During maximal stimulation of acid the parietal cells from the superficial mucosal layers displayed a 40% larger secretory surface than those from the deeper parts of the mucosa. The data, which will serve as a basis for studies of pathological mucosae, are compared with those obtained in other species.
从十名健康男性志愿者的内镜活检中获取了正常人类胃黏膜的定量形态学数据。在静息状态和最大胃酸分泌期间对胃体黏膜进行活检，然后进行光镜和电镜处理。对包含上皮层全层厚度的切片进行体视学分析。黏膜体积的约三分之一由固有层占据，15%由壁细胞占据。对切片中显示细胞核的细胞计数表明，上皮细胞中平均12%为壁细胞，43%为黏液细胞，40%为酶原细胞，4%为内分泌细胞。显示两个细胞核的壁细胞比只有一个细胞核的壁细胞大一倍。壁细胞体积的6%由细胞核占据，细胞质体积的33%被线粒体占据。胃酸分泌刺激导致分泌表面密度增加76%；同时壁细胞的平均大小略有减小，细胞核的相对体积增加。在最大胃酸刺激期间，浅表黏膜层的壁细胞比黏膜深层的壁细胞分泌表面大40%。这些数据将作为病理性黏膜研究的基础，并与其他物种获得的数据进行比较。
