Xing Wen, Hu Ning, Li Zhongfang, Yuan Meng, Luo Meiling, Han Shuo, Blagodatskaya Evgenia, Lu Shunbao, Lou Yilai
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
School of Food and Biological Engineering, Hezhou University, Hezhou 542899, China.
Microorganisms. 2023 Oct 19;11(10):2589. doi: 10.3390/microorganisms11102589.
Selecting the appropriate indicators and measuring time point numbers is important for accurately examining the shift in soil gross decomposition channel structure. Through a selected case study on a natural forest vs. rainfed arable system over a two-month-long experiment, the utility of three commonly employed indicators (fungi to bacteria ratio (F:B), fungivore to bacterivore ratio (FF:BF), and glucosamine to muramic acid ratio (GlcN:MurN)) were compared to reflect the shift in soil gross decomposition channel structure. The requirement of measuring the time point numbers for the three indicators was also assessed, and we suggest a potential methodology. Our results revealed that the GlcN:MurN ratio was more reliable for assessing the shifts in gross decomposition channel structure for long-term land use changes, while it was less sensitive to short-term drought compared with the other two indicators. The F:B ratio was more applicable than the FF:BF ratio for reflecting both long- and short-term changes. Furthermore, the reliability of the GlcN:MurN ratio was the least dependent on measuring time point numbers. We suggest the use of multiple indicators and the adoption of multiple measuring time points for the overall methodology.
选择合适的指标和测量时间点数对于准确考察土壤总分解通道结构的变化至关重要。通过对天然林与旱作耕地系统进行为期两个月的实验案例研究，比较了三种常用指标（真菌与细菌比率（F:B）、食真菌者与食细菌者比率（FF:BF）以及氨基葡萄糖与胞壁酸比率（GlcN:MurN））反映土壤总分解通道结构变化的效用。还评估了这三种指标测量时间点数的要求，并提出了一种潜在方法。我们的结果表明，GlcN:MurN比率在评估长期土地利用变化导致的总分解通道结构变化方面更为可靠，而与其他两个指标相比，它对短期干旱不太敏感。F:B比率比FF:BF比率更适用于反映长期和短期变化。此外，GlcN:MurN比率的可靠性对测量时间点数的依赖程度最低。我们建议在整体方法中使用多种指标并采用多个测量时间点。
