棕色固氮菌脱氧核糖核酸及折叠染色体的特性分析
Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes.
作者信息
Sadoff H L, Shimel B, Ellis S
出版信息
J Bacteriol. 1979 Jun;138(3):871-7. doi: 10.1128/jb.138.3.871-877.1979.
Abstract
The properties of Azotobacter vinelandii deoxyribonucleic acid (DNA) and folded chromosomes were studied and compared to those of Escherichia coli as a standard. Based on melting temperature and buoyant density measurements, the guanosine + cytosine content of purified A. vinelandii DNA was 65%, whereas that of E. coli DNA was 50%. The results of renaturation studies showed that the unique DNA sequence lengths of the two organisms were similar with Cot1/2 values of 7.3 +/- 0.4 mol.s/liter and 7.5 +/- 0.3 mol.s/liter, respectively, for A. vinelandii and E. coli. Folded chromosomes of A. vinelandii sedimented in a centrifugal field at a rate identical to those derived from E. coli, 1,600 to 1,700S. Based on the DNA content per cell and the mass of a single genome, A. vinelandii contains at least 40 chromosomes per cell.
摘要
研究了棕色固氮菌脱氧核糖核酸（DNA）和折叠染色体的特性，并与作为标准的大肠杆菌的特性进行了比较。根据解链温度和浮力密度测量，纯化的棕色固氮菌DNA中鸟嘌呤+胞嘧啶含量为65%，而大肠杆菌DNA的该含量为50%。复性研究结果表明，两种生物的独特DNA序列长度相似，棕色固氮菌和大肠杆菌的Cot1/2值分别为7.3±0.4mol·s/升和7.5±0.3mol·s/升。棕色固氮菌的折叠染色体在离心场中的沉降速率与大肠杆菌的相同，为1600至1700S。根据每个细胞的DNA含量和单个基因组的质量，棕色固氮菌每个细胞至少含有40条染色体。
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