Total Flavonoids from Alleviated -Induced Lung Injury via Inhibition of the TLR2-Mediated NF-κB and MAPK Pathways.

() is an atypical bacterial pathogen responsible for community-acquired pneumonia primarily among school-aged children and young adults. () has been used as a medicinal and edible plant in China for centuries, the constituents from which possessed various bioactivities. Notably, flavonoids existing in residues of defatted seeds exhibited significant anti-inflammatory activities. In the present study, we investigated the impact of total flavonoids from (TFCO) seed extract on pneumonia. TFCO was obtained using multiple column chromatography methods and identified as kaempferol glycosides via UPLC-HRESIMS. In a pneumonia mouse model, TFCO significantly reduced the lung damage, suppressed IL-1β, IL-6, and TNF-α production, and curbed TLR2 activation triggered by . Similarly, in RAW264.7 macrophage cells stimulated by lipid-associated membrane proteins (LAMPs), TFCO suppressed the generation of proinflammatory cytokines and TLR2 expression. Moreover, TFCO diminished the phosphorylation of IκBα, JNK, ERK, p38, and p65 nuclear translocation in vitro. In conclusion, TFCO alleviated -induced lung damage via inhibition of TLR2-mediated NF-κB and MAPK pathways, suggesting its potential therapeutic application in -triggered lung inflammation.