Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of Abel.

BACKGROUND

Abel () is widely cultivated and serves as an important source of edible oil. Yet, during oil production, pruned branches generate significant waste and contribute to environmental pollution.

OBJECTIVES

In this work, we obtain a natural polysaccharide from the branches of and optimize its extraction using Box-Behnken design (BBD), which is a statistical method commonly used in response surface methodology. Additionally, we study its properties, such as monosaccharide composition, structural features, antioxidant, and anti-inflammatory abilities.

RESULTS

BBD was employed to optimize polysaccharide extraction (solid-liquid ratio = 1:40; 90 °C; 130 min) for a higher yield. After purification, the major monosaccharides of branches of polysaccharide (CBP) were disclosed as glucose and galactose. Subsequent structural features of CBP were measured. The antioxidant and anti-inflammatory abilities were measured. The highly scavenging rates of the 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, with the chelating capacity of Fe, indicate potent antioxidant activity of CBP.

CONCLUSIONS

In general, CBP demonstrated significant anti-inflammatory activity with down-regulating the expression of IL-6 and IL-1β in the LPS-induced macrophage RAW264.7 model. This bioactive polysaccharide adds value to waste branches by offering a novel approach to waste recycling and the development of .