Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
Int J Mol Sci. 2023 Oct 11;24(20):15070. doi: 10.3390/ijms242015070.
Tea leaf-color mutants have attracted increasing attention due to their accumulation of quality-related biochemical components. However, there is limited understanding of the molecular mechanisms behind leaf-color bud mutation in tea plants. In this study, a chlorina tea shoot (HY) and a green tea shoot (LY) from the same tea plant were investigated using transcriptome and biochemical analyses. The results showed that the chlorophyll a, chlorophyll b, and total chlorophyll contents in the HY were significantly lower than the LY's, which might have been caused by the activation of several genes related to chlorophyll degradation, such as and . The down-regulation of the , , and involved in flavonoid biosynthesis might result in the reduction in catechins, and the up-regulated and might bring about the accumulation of glutamate in HY. RT-qPCR assays of nine DEGs confirmed the RNA-seq results. Collectively, these findings provide insights into the molecular mechanism of the chlorophyll deficient-induced metabolic change in tea plants.
由于富含与品质相关的生化成分，茶叶叶色突变体受到越来越多的关注。然而，茶树芽叶颜色突变的分子机制仍知之甚少。本研究利用转录组学和生化分析，对来自同一茶树的黄化芽（HY）和绿芽（LY）进行了研究。结果表明，HY 中的叶绿素 a、叶绿素 b 和总叶绿素含量显著低于 LY，这可能是由于几个与叶绿素降解相关的基因，如 和 被激活所致。类黄酮生物合成相关的 、 、 和 下调可能导致儿茶素含量降低，而谷氨酸的积累可能与上调的 和 有关。对 9 个差异表达基因的 RT-qPCR 验证了 RNA-seq 的结果。综上所述，这些发现为阐明茶树中因叶绿素缺乏引起的代谢变化的分子机制提供了新的见解。
