Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Republic of Korea.
Int J Mol Sci. 2023 Oct 11;24(20):15073. doi: 10.3390/ijms242015073.
Monitoring the microenvironment within specific cellular regions is crucial for a comprehensive understanding of life events. Fluorescent probes working in different ranges of pH regions have been developed for the local imaging of different pH environments. Especially, rhodamine-based fluorescent pH probes have been of great interest due to their ON/OFF fluorescence depending on the spirolactam ring's opening/closure. By introducing the -alkyl-hydroxamic acid instead of the alkyl amines in the spirolactam of rhodamine, we were able to tune the pH range where the ring opening and closing of the spirolactam occurs. This six-membered cyclic hydroxamate spirolactam ring of rhodamine B proved to be highly fluorescent in acidic pH environments. In addition, we could monitor pH changes of lysosomes in live cells and zebrafish.
监测特定细胞区域内的微环境对于全面了解生命事件至关重要。已经开发出在不同 pH 区域范围内工作的荧光探针，用于局部成像不同的 pH 环境。特别是基于罗丹明的荧光 pH 探针由于其依赖螺环酰胺环的开/关的 ON/OFF 荧光而备受关注。通过在罗丹明的螺环酰胺中引入 - 烷基 - 羟肟酸而不是烷基胺，我们能够调节螺环酰胺开环和闭环发生的 pH 范围。罗丹明 B 的这种六元环状羟肟酸螺环酰胺环在酸性 pH 环境中表现出高度荧光性。此外，我们可以在活细胞和斑马鱼中监测溶酶体的 pH 变化。
