Department of the Structure and Function of Chromosomes, Laboratory of Molecular Genetics, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.
Laboratory of Gene Engineering, Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia.
Int J Mol Sci. 2023 Oct 11;24(20):15082. doi: 10.3390/ijms242015082.
Hodgkin's lymphomas (HL) and the majority of non-Hodgkin's lymphomas (NHL) derive from different stages of B-cell differentiation. MicroRNA (miRNA) expression profiles change during lymphopoiesis. Thus, miRNA expression analysis can be used as a reliable diagnostic tool to differentiate tumors. In addition, the identification of miRNA's role in lymphopoiesis impairment is an important fundamental task. The aim of this study was to analyze unique miRNA expression profiles in different types of B-cell lymphomas. We analyzed the expression levels of miRNA-18a, -20a, -96, -182, -183, -26b, -34a, -148b, -9, -150, -451a, -23b, -141, and -128 in lymph nodes (LNs) in the following cancer samples: HL (n = 41), diffuse large B-cell lymphoma (DLBCL) (n = 51), mantle cell lymphoma (MCL) (n = 15), follicular lymphoma (FL) (n = 12), and lymphadenopathy (LA) (n = 37), as well as bone marrow (BM) samples: HL (n = 11), DLBCL (n = 42), MCL (n = 14), FL (n = 16), and non-cancerous blood diseases (NCBD) (n = 43). The real-time RT-PCR method was used for analysis. An increase in BM expression levels of miRNA-26b, -150, and -141 in MCL ( < 0.01) and a decrease in BM levels of the miR-183-96-182 cluster and miRNA-451a in DLBCL ( < 0.01) were observed in comparison to NCBD. We also obtained data on increased LN levels of the miR-183-96-182 cluster in MCL ( < 0.01) and miRNA-18a, miRNA-96, and miRNA-9 in FL ( < 0.01), as well as decreased LN expression of miRNA-150 in DLBCL ( < 0.01), and miRNA-182, miRNA-150, and miRNA-128 in HL ( < 0.01). We showed that miRNA expression profile differs between BM and LNs depending on the type of B-cell lymphoma. This can be due to the effect of the tumor microenvironment.
霍奇金淋巴瘤 (HL) 和大多数非霍奇金淋巴瘤 (NHL) 源自 B 细胞分化的不同阶段。微小 RNA (miRNA) 表达谱在淋巴生成过程中发生变化。因此，miRNA 表达分析可用作可靠的诊断工具来区分肿瘤。此外，确定 miRNA 在淋巴生成受损中的作用是一项重要的基础任务。本研究旨在分析不同类型 B 细胞淋巴瘤中独特的 miRNA 表达谱。我们分析了以下癌症样本中淋巴结 (LN) 的 miRNA-18a、-20a、-96、-182、-183、-26b、-34a、-148b、-9、-150、-451a、-23b、-141 和 -128 的表达水平：HL（n = 41）、弥漫性大 B 细胞淋巴瘤 (DLBCL)（n = 51）、套细胞淋巴瘤 (MCL)（n = 15）、滤泡性淋巴瘤 (FL)（n = 12）和淋巴结病 (LA)（n = 37），以及骨髓 (BM) 样本：HL（n = 11）、DLBCL（n = 42）、MCL（n = 14）、FL（n = 16）和非癌性血液病 (NCBD)（n = 43）。使用实时 RT-PCR 方法进行分析。与 NCBD 相比，我们观察到 MCL 中 BM 表达水平的 miRNA-26b、-150 和 -141 增加（<0.01），DLBCL 中 miR-183-96-182 簇和 miRNA-451a 水平降低（<0.01）。我们还获得了 LN 中 MCL 中 miR-183-96-182 簇水平升高（<0.01）和 miRNA-18a、miRNA-96 和 miRNA-9 在 FL 中升高（<0.01）的数据，以及 DLBCL 中 LN 表达降低的 miRNA-150（<0.01），以及 HL 中 miRNA-182、miRNA-150 和 miRNA-128（<0.01）。我们表明，miRNA 表达谱因 B 细胞淋巴瘤的类型而异，在 BM 和 LN 之间存在差异。这可能是由于肿瘤微环境的影响。
