Nowicki Mateusz, Szemraj Janusz, Wierzbowska Agnieszka, Pluta Agnieszka, Grzybowska-Izydorczyk Olga, Nowicka Aleksandra, Stelmach Piotr, Czemerska Magdalena, Szmigielska-Kapłon Anna
Department of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Łódź, Poland.
Department of Medical Biochemistry, Medical University of Lodz, 92-215 Łódź, Poland.
Biology (Basel). 2021 Jul 15;10(7):668. doi: 10.3390/biology10070668.
microRNAs play an important role in the regulation of gene expression, cell fate, hematopoiesis, and may influence the efficacy of CD34+ cell mobilization. The present study examines the role of hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-34a-5p, hsa-miR-126-3p, hsa-miR-146a-5p, hsa-miR-155-5p, and hsa-miR-223-3p in the course of hematopoietic stem cell mobilization. The numbers of CD34+ cells collected in patients with hematological malignancies (39 multiple myelomas, 11 lymphomas) were determined during mobilization for an autologous hematopoietic stem cell transplantation. The miRNA level was evaluated by RT-PCR. Compared to baseline, a significant decline in hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-126-3p, hsa-miR-146a-5p, and hsa-miR-155-5p was observed on the day of the first apheresis (day A). An increase was observed only in the expression of hsa-miR-34a-5p. On day A, a negative correlation was found between hsa-miR-15a-5p and hsa-miR-146a-5p levels and the number of CD34+ cells in peripheral blood. A negative correlation was observed between hsa-miR-146a-5p and the number of collected CD34+ cells after the first apheresis. Good mobilizers, defined according to GITMO criteria, demonstrated a lower hsa-miR-146a-5p level on day A than poor mobilizers. Patients from the hsa-miR-146a-5p "low expressors" collected more CD34+ cells than "high expressors". Our results suggest that the investigated miRNAs, especially hsa-miR-146a-5p, may influence the efficacy of HSC mobilization.
微小RNA在基因表达调控、细胞命运决定、造血过程中发挥着重要作用，并且可能影响CD34+细胞动员的效果。本研究探讨了hsa-miR-15a-5p、hsa-miR-16-5p、hsa-miR-34a-5p、hsa-miR-126-3p、hsa-miR-146a-5p、hsa-miR-155-5p和hsa-miR-223-3p在造血干细胞动员过程中的作用。在进行自体造血干细胞移植动员期间，测定了血液系统恶性肿瘤患者（39例多发性骨髓瘤、11例淋巴瘤）采集的CD34+细胞数量。通过逆转录聚合酶链反应评估微小RNA水平。与基线相比，在首次单采术当天（A日）观察到hsa-miR-15a-5p、hsa-miR-16-5p、hsa-miR-126-3p、hsa-miR-146a-5p和hsa-miR-155-5p显著下降。仅观察到hsa-miR-34a-5p的表达增加。在A日，hsa-miR-15a-5p和hsa-miR-146a-5p水平与外周血中CD34+细胞数量之间存在负相关。在首次单采术后，观察到hsa-miR-146a-5p与采集的CD34+细胞数量之间存在负相关。根据全球造血干细胞动员组织（GITMO）标准定义的良好动员者在A日的hsa-miR-146a-5p水平低于不良动员者。来自hsa-miR-146a-5p“低表达者”的患者比“高表达者”采集到更多的CD34+细胞。我们的结果表明，所研究的微小RNA，尤其是hsa-miR-146a-5p，可能影响造血干细胞动员的效果。
