Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region, Engineering Technology Research Center of Maize Germplasm Resources Innovation on Cold land of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.
Institute of Crop Resources Research, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
Int J Mol Sci. 2023 Oct 12;24(20):15099. doi: 10.3390/ijms242015099.
The NAC gene family has transcription factors specific to plants, which are involved in development and stress response and adaptation. In this study, , an NAC gene in maize that plays a role in saline-alkaline tolerance, was isolated and characterized. ZmNAC89 was localized in the nucleus and had transcriptional activation activity during in vitro experiments. The expression of was strongly upregulated under saline-alkaline, drought and ABA treatments. Overexpression of the gene in transgenic and maize enhanced salt tolerance at the seedling stage. Differentially expressed genes (DEGs) were then confirmed via RNA-sequencing analysis with the transgenic maize line. GO analyses showed that oxidation-reduction process-regulated genes were involved in -mediated salt-alkaline stress. may regulate maize saline-alkali tolerance through the REDOX pathway and ABA signal transduction pathway. From 140 inbred maize lines, 20 haplotypes and 16 SNPs were found in the coding region of the gene, including the excellent haplotype HAP20. These results contribute to a better understanding of the response mechanism of maize to salt-alkali stress and marker-assisted selection during maize breeding.
NAC 基因家族拥有植物特异性转录因子，参与发育以及对胁迫的响应和适应。本研究中，从玉米中分离并鉴定了一个在耐盐碱性方面起作用的 NAC 基因。ZmNAC89 定位于细胞核内，在体外实验中具有转录激活活性。在盐碱性、干旱和 ABA 处理下，的表达强烈上调。过表达该基因在转基因 和玉米中增强了幼苗期的耐盐性。然后通过对转基因玉米系的 RNA-seq 分析确认了差异表达基因（DEGs）。GO 分析表明，氧化还原过程调控的基因参与了 ZmNAC89 介导的盐碱性胁迫。ZmNAC89 可能通过氧化还原途径和 ABA 信号转导途径来调节玉米的耐盐碱性。在 140 个自交系玉米中，在基因的编码区发现了 20 个单倍型和 16 个 SNPs，包括优良单倍型 HAP20。这些结果有助于更好地理解玉米对盐碱性胁迫的响应机制，并在玉米育种中进行标记辅助选择。
