College of Agronomy, Jilin Agricultural University, Changchun 130118, China.
Jilin Academy of Agricultural Sciences, Changchun 130118, China.
Int J Mol Sci. 2022 Nov 1;23(21):13349. doi: 10.3390/ijms232113349.
Osmotic stress caused by drought and high salinity is the key factor limiting plant growth. However, its underlying molecular regulatory mechanism remains unclear. In this study, we found the stress-related gene Zm00001d019704 () based on transcriptome sequencing results previously obtained in the laboratory and determined its biological function in maize. We found that was significantly expressed in both roots and leaves under 10% PEG6000 or 150 mM NaCl. Subcellular localization showed that the gene was localized in the nucleus. The germination rate and root length of the overexpressing lines were significantly increased under drought or salt stress compared with the control. However, after drought stress, the survival rate and relative water content of maize were increased, while the water loss rate was slowed down. Under salt stress, the Na concentration and Na: K ratio of maize was increased. In addition, the contents of antioxidant enzymes and proline in maize under drought or salt stress were higher than those in the control, while the contents of MDA, HO and O were lower than those in the control. The results showed that the gene played its biological function by regulating the ROS signaling pathway. An interaction between ZmSRG7 and the Zmdhn1 protein was found using a yeast two-hybrid experiment. These results suggest that the gene can improve maize tolerance to drought or salt by regulating hydrogen peroxide homeostasis.
渗透胁迫由干旱和高盐引起，是限制植物生长的关键因素。然而，其潜在的分子调控机制尚不清楚。本研究基于实验室先前获得的转录组测序结果，发现了与胁迫相关的基因 Zm00001d019704()，并确定了其在玉米中的生物学功能。我们发现，在 10%PEG6000 或 150mMNaCl 胁迫下，基因在根和叶中均有显著表达。亚细胞定位显示该基因定位于细胞核。与对照相比，过表达株系在干旱或盐胁迫下的发芽率和根长显著增加。然而，在干旱胁迫后，玉米的存活率和相对含水量增加，而水分流失率减缓。在盐胁迫下，玉米的 Na 浓度和 Na:K 比值增加。此外，在干旱或盐胁迫下，玉米中的抗氧化酶和脯氨酸含量高于对照，而 MDA、HO 和 O 的含量低于对照。结果表明，基因通过调节 ROS 信号通路发挥其生物学功能。酵母双杂交实验发现 ZmSRG7 与 Zmdhn1 蛋白之间存在相互作用。这些结果表明，基因可以通过调节过氧化氢稳态来提高玉米对干旱或盐的耐受性。
