State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, China.
Key Open Laboratory of Forest Genetics and Gene Engineering of National Forestry & Grassland, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2023 Oct 14;24(20):15189. doi: 10.3390/ijms242015189.
-methyladenosine (mA) is becoming one of the most important RNA modifications in plant growth and development, including defense, cell differentiation, and secondary metabolism. YT521-B homology (YTH) domain-containing RNA-binding proteins, identified as mA readers in epitranscriptomics, could affect the fate of mA-containing RNA by recognizing and binding the mA site. Therefore, the identification and study of the YTH gene family in () can provide a molecular basis for the study of the role of mA in , but studies on the gene in have not been reported. We identified nine putative gene models in the genome, which can be divided into DF subgroups and DC subgroups. Domain sequence analysis showed that the LcYTH protein had high sequence conservation. A LcYTH aromatic cage bag is composed of tryptophan and tryptophan (WWW). PrLDs were found in the protein results of YTH, suggesting that these genes may be involved in the process of liquid-liquid phase separation. genes have different tissue expression patterns, but the expression of is absolutely dominant in all tissues. In addition, the expression of the genes is changed in response to ABA and MeJA. In this study, We identified and analyzed the expression pattern of genes. Our results laid a foundation for further study of the function of the gene and further genetic and functional analyses of mA RNA modification in forest trees.
N6-甲基腺嘌呤(m6A)是植物生长发育过程中(包括防御、细胞分化和次生代谢)最重要的 RNA 修饰之一。在表观转录组学中被鉴定为 mA 读码器的 YT521-B 同源(YTH)结构域蛋白,通过识别和结合 mA 位点,影响 mA 包含 RNA 的命运。因此,鉴定和研究 ()中的 YTH 基因家族可以为 mA 在 中的作用研究提供分子基础,但尚未有关于 基因的研究报道。我们在 基因组中鉴定了 9 个推定的 基因模型,它们可以分为 DF 亚组和 DC 亚组。结构域序列分析表明,LcYTH 蛋白具有高度的序列保守性。LcYTH 芳香笼袋由色氨酸和色氨酸(WWW)组成。在 YTH 的蛋白质结果中发现了 PrLDs,表明这些基因可能参与液-液相分离过程。 基因具有不同的组织表达模式,但 在所有组织中的表达绝对占优势。此外, 基因的表达在 ABA 和 MeJA 的响应下发生变化。在本研究中,我们鉴定和分析了 基因的表达模式。我们的结果为进一步研究 基因的功能以及树木中 mA RNA 修饰的进一步遗传和功能分析奠定了基础。