• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

在 中全基因组鉴定和表征 YTH 结构域包含的 RNA 结合蛋白家族

Genome-Wide Identification and Characterization of the YTH Domain-Containing RNA-Binding Protein Family in .

机构信息

State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, China.

Key Open Laboratory of Forest Genetics and Gene Engineering of National Forestry & Grassland, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Int J Mol Sci. 2023 Oct 14;24(20):15189. doi: 10.3390/ijms242015189.

DOI:10.3390/ijms242015189
PMID:37894868
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10606907/
Abstract

-methyladenosine (mA) is becoming one of the most important RNA modifications in plant growth and development, including defense, cell differentiation, and secondary metabolism. YT521-B homology (YTH) domain-containing RNA-binding proteins, identified as mA readers in epitranscriptomics, could affect the fate of mA-containing RNA by recognizing and binding the mA site. Therefore, the identification and study of the YTH gene family in () can provide a molecular basis for the study of the role of mA in , but studies on the gene in have not been reported. We identified nine putative gene models in the genome, which can be divided into DF subgroups and DC subgroups. Domain sequence analysis showed that the LcYTH protein had high sequence conservation. A LcYTH aromatic cage bag is composed of tryptophan and tryptophan (WWW). PrLDs were found in the protein results of YTH, suggesting that these genes may be involved in the process of liquid-liquid phase separation. genes have different tissue expression patterns, but the expression of is absolutely dominant in all tissues. In addition, the expression of the genes is changed in response to ABA and MeJA. In this study, We identified and analyzed the expression pattern of genes. Our results laid a foundation for further study of the function of the gene and further genetic and functional analyses of mA RNA modification in forest trees.

摘要

N6-甲基腺嘌呤(m6A)是植物生长发育过程中(包括防御、细胞分化和次生代谢)最重要的 RNA 修饰之一。在表观转录组学中被鉴定为 mA 读码器的 YT521-B 同源(YTH)结构域蛋白,通过识别和结合 mA 位点,影响 mA 包含 RNA 的命运。因此,鉴定和研究 ()中的 YTH 基因家族可以为 mA 在 中的作用研究提供分子基础,但尚未有关于 基因的研究报道。我们在 基因组中鉴定了 9 个推定的 基因模型,它们可以分为 DF 亚组和 DC 亚组。结构域序列分析表明,LcYTH 蛋白具有高度的序列保守性。LcYTH 芳香笼袋由色氨酸和色氨酸(WWW)组成。在 YTH 的蛋白质结果中发现了 PrLDs,表明这些基因可能参与液-液相分离过程。 基因具有不同的组织表达模式,但 在所有组织中的表达绝对占优势。此外, 基因的表达在 ABA 和 MeJA 的响应下发生变化。在本研究中,我们鉴定和分析了 基因的表达模式。我们的结果为进一步研究 基因的功能以及树木中 mA RNA 修饰的进一步遗传和功能分析奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/0e805a4ccc70/ijms-24-15189-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/0592409de846/ijms-24-15189-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/c3cbc125c27d/ijms-24-15189-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/49965fafabe5/ijms-24-15189-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/f75ee98516c0/ijms-24-15189-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/3fd2c36549f2/ijms-24-15189-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/3068f2adb6e7/ijms-24-15189-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/230088dbdbc4/ijms-24-15189-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/0e805a4ccc70/ijms-24-15189-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/0592409de846/ijms-24-15189-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/c3cbc125c27d/ijms-24-15189-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/49965fafabe5/ijms-24-15189-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/f75ee98516c0/ijms-24-15189-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/3fd2c36549f2/ijms-24-15189-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/3068f2adb6e7/ijms-24-15189-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/230088dbdbc4/ijms-24-15189-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b230/10606907/0e805a4ccc70/ijms-24-15189-g008a.jpg

相似文献

1
Genome-Wide Identification and Characterization of the YTH Domain-Containing RNA-Binding Protein Family in .在 中全基因组鉴定和表征 YTH 结构域包含的 RNA 结合蛋白家族
Int J Mol Sci. 2023 Oct 14;24(20):15189. doi: 10.3390/ijms242015189.
2
Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in common wheat.小麦 YTH 结构域包含 RNA 结合蛋白家族的全基因组鉴定和表达分析。
BMC Plant Biol. 2020 Jun 23;20(1):351. doi: 10.1186/s12870-020-02505-1.
3
Genome-Wide Identification and Expression Analysis of Members in the YT521-B Homology Domain-Containing RNA Binding Protein Family in ..中含YT521-B同源结构域的RNA结合蛋白家族成员的全基因组鉴定与表达分析
Plants (Basel). 2024 Dec 23;13(24):3589. doi: 10.3390/plants13243589.
4
Genome-Wide Identification and Expression Analysis of the YTH Domain-Containing RNA-Binding Protein Family in .小麦中 YTH 结构域包含 RNA 结合蛋白家族的全基因组鉴定和表达分析。
Int J Mol Sci. 2024 May 29;25(11):5960. doi: 10.3390/ijms25115960.
5
Structural Basis for the Discriminative Recognition of N6-Methyladenosine RNA by the Human YT521-B Homology Domain Family of Proteins.人类YT521-B同源结构域蛋白家族对N6-甲基腺苷RNA进行特异性识别的结构基础
J Biol Chem. 2015 Oct 9;290(41):24902-13. doi: 10.1074/jbc.M115.680389. Epub 2015 Aug 28.
6
Systematic molecular profiling of non-native N-substitution effects on m6A binding to the YTH domains of human RNA m6A readers in diabetes.糖尿病中关于非天然N-取代对m6A与人类RNA m6A读取器的YTH结构域结合影响的系统性分子分析。
Biophys Chem. 2025 May-Jun;320-321:107417. doi: 10.1016/j.bpc.2025.107417. Epub 2025 Feb 18.
7
Genome-wide identification, characterization, and expression analysis of m6A readers-YTH domain-containing genes in alfalfa.全基因组鉴定、特征分析及苜蓿 m6A 阅读蛋白(YTH 结构域蛋白)基因的表达分析。
BMC Genomics. 2024 Jan 2;25(1):18. doi: 10.1186/s12864-023-09926-w.
8
Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress.中国鹅掌楸 WRKY 基因家族的全基因组鉴定及其在多种非生物胁迫响应中的多样化功能。
BMC Plant Biol. 2022 Jan 10;22(1):25. doi: 10.1186/s12870-021-03371-1.
9
Identification of YTH Domain-Containing Proteins as the Readers for N1-Methyladenosine in RNA.鉴定含有 YTH 结构域的蛋白作为 RNA 中 N1-甲基腺苷的读码器。
Anal Chem. 2018 Jun 5;90(11):6380-6384. doi: 10.1021/acs.analchem.8b01703. Epub 2018 May 25.
10
Genome-wide identification and characterization of YTH domain-containing genes, encoding the mA readers, and their expression in tomato.全基因组鉴定和特征分析 YTH 结构域基因,这些基因编码 mA 读取器,并在番茄中进行其表达研究。
Plant Cell Rep. 2021 Jul;40(7):1229-1245. doi: 10.1007/s00299-021-02716-2. Epub 2021 Jun 3.

引用本文的文献

1
Genome-Wide Identification and Expression Analysis of the YTH Domain-Containing Protein Gene Family in ..中含YTH结构域蛋白基因家族的全基因组鉴定与表达分析
Int J Mol Sci. 2025 May 13;26(10):4645. doi: 10.3390/ijms26104645.
2
Detection, distribution, and functions of RNA -methyladenosine (mA) in plant development and environmental signal responses.RNA甲基腺嘌呤(mA)在植物发育和环境信号应答中的检测、分布及功能
Front Plant Sci. 2024 Jul 16;15:1429011. doi: 10.3389/fpls.2024.1429011. eCollection 2024.
3
Genome-Wide Identification and Expression Analysis of the YTH Domain-Containing RNA-Binding Protein Family in .

本文引用的文献

1
mA readers ECT2/ECT3/ECT4 enhance mRNA stability through direct recruitment of the poly(A) binding proteins in Arabidopsis.mA 读者 ECT2/ECT3/ECT4 通过直接招募拟南芥中的多聚(A)结合蛋白来增强 mRNA 稳定性。
Genome Biol. 2023 Apr 30;24(1):103. doi: 10.1186/s13059-023-02947-4.
2
Detection, regulation, and functions of RNA N-methyladenosine modification in plants.RNA N6-甲基腺苷修饰的检测、调控及功能研究在植物中。
Plant Commun. 2023 May 8;4(3):100546. doi: 10.1016/j.xplc.2023.100546. Epub 2023 Jan 10.
3
Chromatin-associated YTHDC1 coordinates heat-induced reprogramming of gene expression.
小麦中 YTH 结构域包含 RNA 结合蛋白家族的全基因组鉴定和表达分析。
Int J Mol Sci. 2024 May 29;25(11):5960. doi: 10.3390/ijms25115960.
4
Genome-Wide Identification and Expression Analysis of Gene Family for Abiotic Stress Regulation in .在 中,对非生物胁迫调节基因家族进行全基因组鉴定和表达分析。
Int J Mol Sci. 2024 Apr 3;25(7):3996. doi: 10.3390/ijms25073996.
染色质相关 YTHDC1 协调热诱导的基因表达重编程。
Cell Rep. 2022 Dec 13;41(11):111784. doi: 10.1016/j.celrep.2022.111784.
4
The RNA N -methyladenosine demethylase ALKBH9B modulates ABA responses in Arabidopsis.RNA N6-甲基腺嘌呤去甲基化酶 ALKBH9B 调控拟南芥 ABA 响应。
J Integr Plant Biol. 2022 Dec;64(12):2361-2373. doi: 10.1111/jipb.13394. Epub 2022 Nov 22.
5
Dynamic control of chromatin-associated mA methylation regulates nascent RNA synthesis.动态控制染色质相关 mA 甲基化调节新生 RNA 合成。
Mol Cell. 2022 Mar 17;82(6):1156-1168.e7. doi: 10.1016/j.molcel.2022.02.006. Epub 2022 Feb 25.
6
N-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation.mRNA 上的 N6-甲基腺苷有助于形成一个液-液相分离的核体,从而抑制髓系白血病的分化。
Cancer Cell. 2021 Jul 12;39(7):958-972.e8. doi: 10.1016/j.ccell.2021.04.017. Epub 2021 May 27.
7
Arabidopsis N-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies.拟南芥 N6-甲基腺苷阅读蛋白 CPSF30-L 识别 FUE 信号,以控制液滴状核体内的多聚腺苷酸化位点选择。
Mol Plant. 2021 Apr 5;14(4):571-587. doi: 10.1016/j.molp.2021.01.014. Epub 2021 Jan 27.
8
Epitranscriptomic regulation by mA RNA methylation in brain development and diseases.m6A RNA 甲基化在脑发育和疾病中的转录后调控作用。
J Cereb Blood Flow Metab. 2020 Dec;40(12):2331-2349. doi: 10.1177/0271678X20960033. Epub 2020 Sep 23.
9
Recurrent requirement for the mA-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis.在植物器官发生过程中控制细胞增殖时,mA-ECT2/ECT3/ECT4 轴反复出现的需求。
Development. 2020 Jul 24;147(14):dev189134. doi: 10.1242/dev.189134.
10
TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data.TBtools:一个用于生物大数据交互式分析的集成工具包。
Mol Plant. 2020 Aug 3;13(8):1194-1202. doi: 10.1016/j.molp.2020.06.009. Epub 2020 Jun 23.