Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159C, 02-776 Warsaw, Poland.
Laboratory of Nanomaterials and Nanotechnology, Faculty of Food Technology, University of Agriculture in Cracow, Balicka Street 122, 30-149 Cracow, Poland.
Int J Mol Sci. 2023 Oct 15;24(20):15204. doi: 10.3390/ijms242015204.
In recent years, the production of plasma-treated water (PTW) by low-temperature low-pressure glow plasma (LPGP) has been increasingly gaining in popularity. LPGP-treated water changes its physical and physiochemical properties compared to standard distilled water. In this study, a non-conventional lipolytic yeast species was cultivated in culture media based on Nantes plasma water with heightened singlet oxygen content (Nantes PW) or in water treated with low-temperature, low-pressure glow plasma while in contact with air (PWTA) or nitrogen (PWTN). The research aimed to assess the influence of culture conditions on castor oil biotransformation to gamma-decalactone (GDL) and other secondary metabolites in media based on nanowater. The Nantes plasma water-based medium attained the highest concentration of gamma-decalactone (4.81 ± 0.51 g/L at 144 h of culture), maximum biomass concentration and biomass yield from the substrate. The amplified activity of lipases in the nanowater-based medium, in comparison to the control medium, is encouraging from the perspective of GDL biosynthesis, relying on the biotransformation of ricinoleic acid, which is the primary component of castor oil. Although lipid hydrolysis was enhanced, this step seemed not crucial for GDL concentration. Interestingly, the study validates the significance of oxygen in β-oxidation enzymes and its role in the bioconversion of ricinoleic acid to GDL and other lactones. Specifically, media with higher oxygen content (WPTA) and Nantes plasma water resulted in remarkably high concentrations of four lactones: gamma-decalactone, 3-hydroxy-gamma-decalactone, dec-2-en-4-olide and dec-3-en-4-olide.
近年来，通过低温低压辉光等离子体（LPGP）生产等离子体处理水（PTW）的方法越来越受到关注。与标准蒸馏水相比，LPGP 处理过的水会改变其物理和物理化学性质。在这项研究中，一种非常规的脂肪酶酵母在基于富含单重态氧的南特等离子体水（Nantes PW）或与空气（PWTA）或氮气（PWTN）接触的低温低压辉光等离子体处理水中的培养基中进行培养。研究旨在评估培养条件对蓖麻油生物转化为γ-癸内酯（GDL）和纳米水基培养基中其他次生代谢物的影响。南特等离子体水基培养基达到了最高的γ-癸内酯浓度（培养 144 小时时为 4.81±0.51g/L）、最大的生物量浓度和底物的生物量产率。与对照培养基相比，纳米水基培养基中脂肪酶的活性增强，这对于 GDL 生物合成是令人鼓舞的，因为它依赖于蓖麻油的主要成分——蓖麻酸的生物转化。尽管脂质水解增强，但这一步似乎对 GDL 浓度并不关键。有趣的是，该研究验证了氧气在β-氧化酶中的重要性及其在蓖麻酸转化为 GDL 和其他内酯中的作用。具体来说，含氧量较高的培养基（WPTA）和南特等离子体水产生了四种内酯的高浓度：γ-癸内酯、3-羟基-γ-癸内酯、癸-2-烯-4-内酯和癸-3-烯-4-内酯。
