State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266237, China.
Int J Mol Sci. 2023 Oct 17;24(20):15278. doi: 10.3390/ijms242015278.
Dopamine receptors (DARs) are important transmembrane receptors responsible for receiving extracellular signals in the DAR-mediated signaling pathway, and are involved in a variety of physiological functions. Herein, the D1 DAR gene from () was identified and characterized. The protein encoded by has the typical structure and functional domains of the G-protein coupled receptor family. expression was significantly upregulated in the gills and hepatopancreas after low temperature stress. Moreover, double-stranded RNA-mediated silencing of significantly changed the levels of protein kinases (PKA and PKC), second messengers (cyclic AMP (cAMP), cyclic cGMP, calmodulin, and diacyl glycerol), and G-protein effectors (adenylate cyclase and phospholipase C). Furthermore, silencing increased the apoptosis rate of gill and hepatopancreas cells. Thus, following binding to their specific receptors, G-protein effectors are activated by , leading to DAD1-cAMP/PKA pathway-mediated regulation of caspase-dependent mitochondrial apoptosis. We suggest that MjDAD1 is indispensable for the environmental adaptation of .
多巴胺受体（DARs）是重要的跨膜受体，负责接收 DAR 介导的信号通路中的细胞外信号，并参与多种生理功能。在此，从（）中鉴定和表征了 D1 DAR 基因。所编码的蛋白具有 G 蛋白偶联受体家族的典型结构和功能域。低温应激后， 在鳃和肝胰腺中的表达显著上调。此外，双链 RNA 介导的 沉默显著改变了蛋白激酶（PKA 和 PKC）、第二信使（环腺苷酸（cAMP）、环鸟苷酸（cGMP）、钙调蛋白和二酰基甘油）和 G 蛋白效应物（腺苷酸环化酶和磷脂酶 C）的水平。此外， 沉默增加了鳃和肝胰腺细胞的凋亡率。因此， 与它们的特定受体结合后，G 蛋白效应物被 激活，导致 DAD1-cAMP/PKA 通路介导的 caspase 依赖性线粒体凋亡的调节。我们认为 MjDAD1 对于 的环境适应是不可或缺的。
