Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China.
Int J Mol Sci. 2023 Oct 22;24(20):15440. doi: 10.3390/ijms242015440.
Species within the genus are valued for their draft ability. Skeletal muscle forms the foundation of the draft ability of species; however, skeletal muscle development-related conserved genes and their target miRNAs are rarely reported for . In this study, a comparative genomics analysis was performed among five species (horse, donkey, zebra, cattle, and goat), and the results showed that a total of 15,262 (47.43%) genes formed the core gene set of the five species. Only nine chromosomes (Chr01, Chr02, Chr03, Chr06, Chr10, Chr18, Chr22, Chr27, Chr29, and Chr30) exhibited a good collinearity relationship among species. The micro-synteny analysis results showed that was evolutionarily conserved in chromosome 1 in . Furthermore, donkeys were used as the model species for to investigate the genetic role of in muscle development. Interestingly, the results of comparative transcriptomics showed that the gene was differentially expressed in donkey skeletal muscle S1 (2 months old) and S2 (24 months old), as verified via RT-PCR. Dual-luciferase test analysis showed that the gene was targeted by differentially expressed miRNA (eca-miR-1). Furthermore, a total of 17 gene family members were identified in the whole genome of donkey, and a heatmap analysis showed that was a key member of the gene family, which is involved in skeletal muscle development. In conclusion, the gene was conserved in , and was targeted by eca-miR-1, which is involved in skeletal muscle development in donkeys.
属于中的物种因其拖曳能力而受到重视。骨骼肌构成了物种拖曳能力的基础；然而，对于 物种，很少有骨骼肌发育相关的保守基因及其靶 miRNAs 被报道。在这项研究中，对五个物种（马、驴、斑马、牛和羊）进行了比较基因组学分析，结果表明，共有 15262 个（47.43%）基因形成了五个物种的核心基因集。只有九条染色体（Chr01、Chr02、Chr03、Chr06、Chr10、Chr18、Chr22、Chr27、Chr29 和 Chr30）在物种间表现出良好的共线性关系。微同线性分析结果表明，在 中，1 号染色体在 中是进化保守的。此外，驴被用作 物种的模型来研究 基因在肌肉发育中的遗传作用。有趣的是，比较转录组学的结果表明，在驴骨骼肌 S1（2 个月大）和 S2（24 个月大）中， 基因的表达存在差异，这一点通过 RT-PCR 得到了验证。双荧光素酶试验分析表明，差异表达的 miRNA（eca-miR-1）靶向 基因。此外，在驴的全基因组中鉴定出了 17 个 基因家族成员，热图分析表明 是 基因家族的关键成员，参与骨骼肌发育。总之，在 中， 基因是保守的，而 eca-miR-1 靶向 基因，该基因参与驴的骨骼肌发育。
