Department of Internal Medicine, College of Medicine, Yeungnam University, 170 Hyunchung-ro, Nam-gu, Daegu 42415, Republic of Korea.
Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea.
Genes (Basel). 2023 Oct 20;14(10):1963. doi: 10.3390/genes14101963.
The clinical significance of hsa_circ_0004018 and hsa_circ_0003570 in patients with hepatitis B virus-related hepatocellular carcinoma (HBV-HCC) is unclear. We aimed to explore the clinical significance and prognostic utility of these two circular RNAs (circRNAs) in patients with HBV-HCC. Based on 86 paired tissue samples of HCC and adjacent non-HCC, the relative expression profiles of hsa_circ_0004018 and hsa_circ_0003570 were determined using quantitative real-time polymerase chain reactions. The cut-off values were the median expression of each of the two circRNAs in 86 patients with HBV-HCC. The combination group comprised patients with high levels of the two circRNAs. Clinicopathological features, body composition profiles at the L3 level, and survival rates were investigated. The expression of hsa_circ_0004018 and hsa_circ_0003570 was downregulated in HCC tissues compared with non-HCC tissues. High expression levels of hsa_circ_0003570 (hazard ratio (HR), 0.437; = 0.009) and hsa_circ_0004018 (HR, 0.435; = 0.005) were inversely independent risk factors for overall and progression-free survival in patients with HBV-HCC, whereas the combination group was also an inversely independent risk factor for overall (HR, 0.399; = 0.005) and progression-free survival (HR, 0.422; = 0.003) in patients with HBV-HCC. The combination of hsa_circ_0003570 and hsa_circ_0004018 may be a potential prognostic biomarker for HBV-HCC.
hsa_circ_0004018 和 hsa_circ_0003570 在乙型肝炎病毒相关肝细胞癌（HBV-HCC）患者中的临床意义尚不清楚。本研究旨在探讨这两种环状 RNA（circRNA）在 HBV-HCC 患者中的临床意义和预后价值。基于 86 对 HCC 和相邻非 HCC 组织样本，采用实时定量聚合酶链反应检测 hsa_circ_0004018 和 hsa_circ_0003570 的相对表达谱。以 86 例 HBV-HCC 患者的两种 circRNA 的中位数表达作为截断值。联合组包括两个 circRNA 水平高的患者。分析了临床病理特征、L3 水平的身体成分谱和生存率。与非 HCC 组织相比，HCC 组织中 hsa_circ_0004018 和 hsa_circ_0003570 的表达下调。hsa_circ_0003570（风险比（HR），0.437；P=0.009）和 hsa_circ_0004018（HR，0.435；P=0.005）高表达是 HBV-HCC 患者总生存和无进展生存的独立负向预后因素，而联合组也是 HBV-HCC 患者总生存（HR，0.399；P=0.005）和无进展生存（HR，0.422；P=0.003）的独立负向预后因素。hsa_circ_0003570 和 hsa_circ_0004018 的联合可能是 HBV-HCC 的一个潜在预后生物标志物。
