Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu, 41944, Republic of Korea.
Department of Surgery, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, 130 Dongdeok-ro, Jung-gu, Daegu, 41944, Republic of Korea.
Sci Rep. 2021 Jul 22;11(1):14930. doi: 10.1038/s41598-021-94074-y.
Circular RNAs (circRNAs) represent potential biomarkers because of their highly stable structure and robust expression pattern in clinical samples. The aim of this study was to evaluate the expression of a recently identified circRNA, hsa_circ_0005986; determine its clinical significance; and evaluate its potential as a biomarker of hepatocellular carcinoma (HCC). We evaluated hsa_circ_0005986 expression in 123 HCC tissue samples, its clinical significance, and its association with patients' clinicopathological characteristics and survival. Hsa_circ_0005986 expression was downregulated in HCC tissues. Low hsa_circ_0005986 expression was more common in tumors larger than 5 cm [odds ratio (OR), 3.19; 95% confidence interval (CI), 1.51-6.76; p = 0.002], advanced TNM stage (III/IV; OR, 2.39; 95% CI, 1.16-4.95; p = 0.018), and higher BCLC stage (B/C; OR, 2.71; 95% CI, 1.30-5.65; p = 0.007). High hsa_circ_0005986 expression was associated with improved survival and was an independent prognostic factor for overall [hazard ratio (HR), 0.572; 95% CI, 0.339-0.966; p = 0.037] and progression-free (HR, 0.573; 95% CI, 0.362-0.906; p = 0.017) survival. Moreover, the circRNA-miRNA-mRNA network was constructed using RNA-seq/miRNA-seq data and clinical information from TCGA-LIHC dataset. Our findings indicate a promising role for hsa_circ_0005986 as a prognostic biomarker in patients with HCC.
环状 RNA（circRNA）因其在临床样本中具有高度稳定的结构和稳健的表达模式，成为有潜力的生物标志物。本研究旨在评估最近发现的环状 RNA hsa_circ_0005986 的表达情况；确定其临床意义；并评估其作为肝细胞癌（HCC）生物标志物的潜力。我们评估了 123 例 HCC 组织样本中 hsa_circ_0005986 的表达情况、其临床意义以及与患者临床病理特征和生存的关系。hsa_circ_0005986 在 HCC 组织中表达下调。在肿瘤大于 5cm（比值比（OR），3.19；95%置信区间（CI），1.51-6.76；p=0.002）、晚期 TNM 分期（III/IV；OR，2.39；95%CI，1.16-4.95；p=0.018）和更高的 BCLC 分期（B/C；OR，2.71；95%CI，1.30-5.65；p=0.007）的肿瘤中，低表达 hsa_circ_0005986 的情况更为常见。高 hsa_circ_0005986 表达与生存改善相关，并且是总生存期（HR，0.572；95%CI，0.339-0.966；p=0.037）和无进展生存期（HR，0.573；95%CI，0.362-0.906；p=0.017）的独立预后因素。此外，还使用 TCGA-LIHC 数据集的 RNA-seq/miRNA-seq 数据和临床信息构建了 circRNA-miRNA-mRNA 网络。我们的研究结果表明，hsa_circ_0005986 作为 HCC 患者的预后生物标志物具有广阔的应用前景。
