Hormozi Maryam, Gholami Mohammadreza, Babaniazi Ayda, Gharravi Anneh Mohammad
1Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
2Department of Biochemistry, Lorestan University of Medical Science, Khorramabad, Iran.
Inflamm Regen. 2019 Apr 20;39:7. doi: 10.1186/s41232-019-0097-x. eCollection 2019.
TGF-β has an important role in the process of wound healing and scar formation. The aim of this study is to determine the effects of ethanolic and methanolic extracts of on the expression of TGFβ1 and bFGF in the mouse embryonic fibroblast cells (MEFs).
extract was purchased and different substances defined with gas chromatography and mass spectrometry. MEFs were prepared and after incubating for 15 min, cell viability analyzed. TGF β 1 and bFGF gene expression was evaluated by real-time PCR. TGFβ1 and bFGF protein expression analyzed by ELISA. The statistical analysis of data was done by using SPSS software. Differences were considered significant at ( < 0.05).
The results of the MTT test showed that the concentrations of 5 μg/ml and10 μg/ml were more suitable for cell proliferation. There was an increase in TGF β 1 gene expression in the MEFs. Expression of TGF β 1 gene remains the same after 24 h. Gene expression of bFGF showed a similar pattern with β expression for both solvents. Analysis of TGFβ1 protein expression showed an increase in TGFβ1 gene expression in the MEFs. Protein expression of bFGF in the MEFs increased at different concentrations at 12 and 24 h after treatment ( < 0.05 and < 0.01 respectively).
stimulates proliferation of MEFs. Calendula via increased expression of growth factors (TGFβ1 and bFGF) at the first 12 h and a decrease of these factors at 24 h after treatment may ameliorate function of the MEFs in the during wound healing.
转化生长因子-β（TGF-β）在伤口愈合和瘢痕形成过程中起重要作用。本研究旨在确定[提取物名称]乙醇提取物和甲醇提取物对小鼠胚胎成纤维细胞（MEFs）中TGFβ1和碱性成纤维细胞生长因子（bFGF）表达的影响。
购买[提取物名称]提取物，并用气相色谱和质谱法确定不同成分。制备MEFs，孵育15分钟后分析细胞活力。通过实时聚合酶链反应（PCR）评估TGF β 1和bFGF基因表达。用酶联免疫吸附测定（ELISA）分析TGFβ1和bFGF蛋白表达。使用社会科学统计软件包（SPSS）软件对数据进行统计分析。差异在P<0.05时被认为具有统计学意义。
噻唑蓝（MTT）试验结果表明，5μg/ml和10μg/ml的浓度更适合细胞增殖。MEFs中TGF β 1基因表达增加。24小时后TGF β 1基因表达保持不变。两种溶剂处理下，bFGF的基因表达与TGF β 1表达呈现相似模式。TGFβ1蛋白表达分析显示MEFs中TGFβ1基因表达增加。处理后12小时和24小时，MEFs中bFGF的蛋白表达在不同浓度下均增加（分别为P<0.05和P<0.01）。
[提取物名称]可刺激MEFs增殖。金盏花可能通过在处理后最初12小时增加生长因子（TGFβ1和bFGF）的表达以及在24小时降低这些因子的表达来改善伤口愈合期间MEFs的功能。
