State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China; School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Bioresour Technol. 2021 Mar;324:124679. doi: 10.1016/j.biortech.2021.124679. Epub 2021 Jan 8.
This work aimed to study the effects of biochar on methanogenesis and antibiotic resistance genes (ARGs) fate during anaerobic digestion (AD) of swine manure (SM) was investigated. Under the optimal biochar dosage of 5-10%, methane yield was significantly improved by 25%, ascribing to the enhancement of direct interspecies electron transfer (DIET). Biochar addition alleviated the need of cytochrome-c as interspecies electron connection components and enriched the microbes involved in DIET. Defluviitoga, Thermovirga and Cloacibacillus were dominant with biochar addition and might participate in DIET together with Methanothrix. The abundance of parC, tetX, bla, bla, aac(6')-Ib-cr, ermB and tetW were significantly reduced in biochar treatments and intI1 removal increased by 15% in B-5% than the control. Structural equation models demonstrated that intI1 (λ = 0.59, P < 0.001) had the most standardized direct effects on ARGs, while biochar indirectly affected ARGs by changing intI1 (λ = 0.41, P < 0.01) and microbial structure (λ = -0.24, P < 0.05).
本研究旨在探讨生物炭对猪粪厌氧消化（AD）中产甲烷和抗生素抗性基因（ARGs）命运的影响。在最优生物炭投加量为 5-10%时，甲烷产量显著提高了 25%，这归因于直接种间电子转移（DIET）的增强。生物炭的添加减轻了细胞色素-c 作为种间电子连接组件的需求，并丰富了参与 DIET 的微生物。添加生物炭后，脱硫弧菌属、热沃氏菌属和栖热菌属占优势，可能与产甲烷菌属一起参与 DIET。与对照组相比，添加生物炭处理后，parC、tetX、bla、bla、aac(6')-Ib-cr、ermB 和 tetW 的丰度显著降低，B-5%组的 intI1 去除率增加了 15%。结构方程模型表明，intI1（λ=0.59，P<0.001）对 ARGs 具有最标准化的直接影响，而生物炭通过改变 intI1（λ=0.41，P<0.01）和微生物结构（λ=-0.24，P<0.05）间接影响 ARGs。
