Alhamhoom Yahya, Ahmed Syed Sagheer, M Rupesh Kumar, Salahuddin M D, D R Bharathi, Ahmed Mohammed Muqtader, Farhana Syeda Ayesha, Rahamathulla Mohamed
Department of Pharmaceutics, College of Pharmacy, King Khalid University, Al Faraa, Abha 62223, Saudi Arabia.
Department of Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, BG Nagara, Mandya 571448, India.
Pharmaceuticals (Basel). 2023 Sep 27;16(10):1368. doi: 10.3390/ph16101368.
Polyherbal formulation (PHF) enhances therapeutic efficacy and minimizes side effects by reducing individual herb dosages. Allopolyherbal formulation (APHF) combines polyherbal extracts with allopathic medication, effectively reducing the latter's required dose and mitigating associated adverse effects. The current study intends to assess the anti-diabetic effects of PHF and APHF . Dried raw powders of leaf, leaf, and rhizome were extracted by cold maceration process using 70% ethanol. These extracts were combined in three different ratios to make PHF. PHF was subjected to qualitative and quantitative phytochemical investigations. APHF has been prepared by combining a potent ratio of PHF with metformin in three different ratios. The compatibility of APHF has been confirmed by differential scanning calorimetry (DSC). In vivo activity was also evaluated in streptozotocin-induced diabetic albino rats. PHF (3 different ratios at a dose of 200-400 mg/kg b.w), APHF (combination of PHF and metformin in 3 different ratios, 200 + 22.5, 200 + 45, and 200 + 67.5 mg/kg b.w), and metformin (90 mg/kg b.w) were administered to albino rats for 21 consecutive days. Blood glucose levels were estimated on the 1st, 7th, 14th, and 21st days of treatment. On the 21st day, blood was collected by cardiac puncture for biochemical analysis. The liver and pancreas were isolated and subjected to histopathological analysis. PHF and APHF showed significant anti-diabetic and antihyperlipidemic efficacy. In comparison to PHF, APHF had the most promising action. The current study demonstrated that PHF and APHF are safe and efficacious drugs in the treatment of diabetes mellitus as they help to replace or lower the dose of metformin, thereby decreasing the risks of metformin.
多草药配方（PHF）通过降低单一草药剂量提高治疗效果并将副作用降至最低。异源多草药配方（APHF）将多草药提取物与对抗疗法药物相结合，有效降低后者所需剂量并减轻相关不良反应。本研究旨在评估PHF和APHF的抗糖尿病作用。使用70%乙醇通过冷浸法提取叶、叶和根茎的干燥生粉。这些提取物按三种不同比例混合制成PHF。对PHF进行了定性和定量植物化学研究。通过将有效比例的PHF与二甲双胍按三种不同比例混合制备了APHF。通过差示扫描量热法（DSC）证实了APHF的相容性。还在链脲佐菌素诱导的糖尿病白化大鼠中评估了体内活性。将PHF（三种不同比例，剂量为200 - 400 mg/kg体重）、APHF（PHF与二甲双胍按三种不同比例混合，200 + 22.5、200 + 45和200 + 67.5 mg/kg体重）和二甲双胍（90 mg/kg体重）连续21天给予白化大鼠。在治疗的第1、7、14和21天估计血糖水平。在第21天，通过心脏穿刺采血进行生化分析。分离肝脏和胰腺并进行组织病理学分析。PHF和APHF显示出显著的抗糖尿病和抗高血脂功效。与PHF相比，APHF的作用最有前景。本研究表明，PHF和APHF在治疗糖尿病方面是安全有效的药物，因为它们有助于替代或降低二甲双胍的剂量，从而降低二甲双胍的风险。
