Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore, India.
Department of Zoology, Annamalai University, Chidambaram, India.
J Diabetes. 2018 Jan;10(1):28-42. doi: 10.1111/1753-0407.12554. Epub 2017 May 29.
Medicinal plants and green synthesis of silver nanoparticles (AgNPs) have proven to be good sources of agents effective in the treatment of diabetes mellitus. The present study focused on the green synthesis of AgNPs from the aqueous leaf extract of Pouteria sapota in order to evaluate the in vitro and in vivo antidiabetic properties of this extract and the synthesized AgNPs.
The AgNPs were biologically synthesized under ambient conditions from an aqueous leaf extract of P. sapota using the hot percolation method and were characterized using spectroscopic methods, X-ray diffraction, and scanning electron microscopy. The in vitro antidiabetic activity of the aqueous leaf extract and AgNPs was confirmed by non-enzymatic glycosylation of hemoglobin, glucose uptake by yeast cells following exposure of cells to 5 or 10 mmol/L glucose solution, and inhibition of α-amylase. Further, in vivo antidiabetic activity was assessed in streptozotocin-induced rats. Rats were treated with aqueous leaf extract (100 mg/kg) or AgNPs (10 mg/kg) for 28 days. Following treatment, rats were killed for biochemical and histopathological analysis of kidney and liver samples.
A significant reduction in blood sugar levels was noted in rats treated with leaf extract or AgNPs. Results of in vitro and in vivo analyses in rats treated with leaf extract or AgNPs show that both the extract and the biologically synthesized AgNPs have antidiabetic activity.
The aqueous leaf extract of P. sapota and AgNPs exhibited efficient antidiabetic activity in the rat model of diabetes and therefore could have potential for development for medical applications in the future.
药用植物和银纳米粒子（AgNPs）的绿色合成已被证明是治疗糖尿病的有效药物的良好来源。本研究专注于从番石榴叶的水提物中绿色合成 AgNPs，以评估该提取物和合成 AgNPs 的体外和体内抗糖尿病特性。
AgNPs 是通过在环境条件下使用热水渗滤法从番石榴叶的水提物中生物合成的，并通过光谱法、X 射线衍射和扫描电子显微镜进行了表征。水提物和 AgNPs 的体外抗糖尿病活性通过血红蛋白的非酶糖化、细胞暴露于 5 或 10mmol/L 葡萄糖溶液后酵母细胞对葡萄糖的摄取以及α-淀粉酶的抑制来证实。此外，还在链脲佐菌素诱导的大鼠中评估了体内抗糖尿病活性。大鼠用番石榴叶提取物（100mg/kg）或 AgNPs（10mg/kg）处理 28 天。治疗后，处死大鼠以进行肾和肝组织样本的生化和组织病理学分析。
用叶提取物或 AgNPs 治疗的大鼠血糖水平显著降低。用叶提取物或 AgNPs 治疗的大鼠的体外和体内分析结果表明，提取物和生物合成的 AgNPs 均具有抗糖尿病活性。
番石榴叶的水提物和 AgNPs 在糖尿病大鼠模型中表现出有效的抗糖尿病活性，因此将来可能有潜力用于医学应用。
