Kim Ji Min, Jung In A, Kim Jae Min, Choi Moon-Hee, Yang Ji Hye
College of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea.
Department of Biochemical Engineering, College of Engineering, Chosun University, Gwangju 61452, Republic of Korea.
Pharmaceuticals (Basel). 2023 Oct 3;16(10):1402. doi: 10.3390/ph16101402.
Siebold (CJ) branch bark, commonly known as Japanese cinnamon, has been used for various culinary and medicinal applications for many centuries. Although the efficacy of CJ branch bark's anti-inflammatory and antioxidant activity for the treatment of various diseases has been confirmed, the efficacy of CJ leaves (CJLs) has not been examined. We therefore investigated whether CJL3, an ethyl acetate extract of a 70% ethanol CJL extract, exerts anti-inflammatory effects on lipopolysaccharide (LPS)-activated Kupffer cells, specialized macrophages found in the liver. Liver inflammation can activate Kupffer cells, inducing the release of pro-inflammatory molecules that contribute to tissue damage. We found that CJL3 has high 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical-scavenging activity. Among the CJL extracts, CJL3 exhibited the greatest polyphenol content, with protocatechuic acid and 4-hydroxybenzoic acid being the most abundant. In addition, we verified that CJL3, which has strong antioxidant properties, ameliorates LPS-induced pro-inflammatory responses by inhibiting p38/JNK/AP-1 signaling. CJL3 therefore has potential for treating liver disease, including hepatitis.
Siebold（CJ）树枝皮，俗称日本肉桂，几个世纪以来一直用于各种烹饪和药用。尽管CJ树枝皮的抗炎和抗氧化活性对治疗各种疾病的功效已得到证实，但CJ叶（CJL）的功效尚未得到研究。因此，我们研究了CJL3（一种70%乙醇CJL提取物的乙酸乙酯提取物）对脂多糖（LPS）激活的库普弗细胞（肝脏中特化的巨噬细胞）是否具有抗炎作用。肝脏炎症可激活库普弗细胞，诱导促炎分子释放，导致组织损伤。我们发现CJL3具有较高的2,2-二苯基-1-苦基肼和2,2-联氮-双（3-乙基苯并噻唑啉-6-磺酸）自由基清除活性。在CJL提取物中，CJL3的多酚含量最高，原儿茶酸和4-羟基苯甲酸含量最为丰富。此外，我们证实，具有强抗氧化特性的CJL3通过抑制p38/JNK/AP-1信号传导减轻LPS诱导的促炎反应。因此，CJL3有治疗包括肝炎在内的肝脏疾病的潜力。
