Nazari Seyedeh Elnaz, Tarnava Alex, Khalili-Tanha Nima, Darroudi Mahdieh, Khalili-Tanha Ghazaleh, Avan Amir, Khazaei Majid, LeBaron Tyler W
Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad 13131-99137, Iran.
Drink HRW, New Westminster, BC V3J 0B6, Canada.
Pharmaceuticals (Basel). 2023 Oct 10;16(10):1436. doi: 10.3390/ph16101436.
Skeletal muscle atrophy is associated with poor quality of life and disability. Thus, finding a new strategy for the prevention and treatment of skeletal muscle atrophy is very crucial. This study aimed to investigate the therapeutic potential of hydrogen-rich water (HRW) on muscle atrophy in a unilateral hind limb immobilization model. Thirty-six male Balb/C mice were divided into control (without immobilization), atrophy, and atrophy + hydrogen-rich water (HRW). Unilateral hind limb immobilization was induced using a splint for 7 days (atrophy) and removed for 10 days (recovery). At the end of each phase, gastrocnemius and soleus muscle weight, limb grip strength, skeletal muscle histopathology, muscle fiber size, cross-section area (CSA), serum troponin I and skeletal muscle IL-6, TNF-α and Malondialdehyde (MDA), and mRNA expression of NF-κB, BAX and Beclin-1 were evaluated. Muscle weight and limb grip strength in the H-treated group were significantly improved during the atrophy phase, and this improvement continued during the recovery period. Treatment by HRW increased CSA and muscle fiber size and reduced muscle fibrosis, serum troponin I, IL-6, TNF-α and MDA which was more prominent in the atrophy phase. These data suggest that HRW could improve muscle atrophy in an immobilized condition and could be considered a new strategy during rehabilitation.
骨骼肌萎缩与生活质量差和残疾相关。因此，寻找预防和治疗骨骼肌萎缩的新策略至关重要。本研究旨在探讨富氢水（HRW）对单侧后肢固定模型中肌肉萎缩的治疗潜力。将36只雄性Balb/C小鼠分为对照组（未固定）、萎缩组和萎缩+富氢水（HRW）组。使用夹板诱导单侧后肢固定7天（萎缩期），然后解除固定10天（恢复期）。在每个阶段结束时，评估腓肠肌和比目鱼肌重量、肢体握力、骨骼肌组织病理学、肌纤维大小、横截面积（CSA）、血清肌钙蛋白I以及骨骼肌白细胞介素-6、肿瘤坏死因子-α和丙二醛（MDA），以及核因子-κB、BAX和Beclin-1的mRNA表达。在萎缩期，HRW治疗组的肌肉重量和肢体握力显著改善，且这种改善在恢复期持续存在。HRW治疗增加了CSA和肌纤维大小，并减少了肌肉纤维化、血清肌钙蛋白I、白细胞介素-6、肿瘤坏死因子-α和MDA，这在萎缩期更为明显。这些数据表明，HRW可以改善固定状态下的肌肉萎缩，并可被视为康复过程中的一种新策略。
