Ran Jiajun, Ding Qiang, Wang Guangpeng, Shen Yunlou, Gao Zhanyuan, Gao Yue, Ma Xiaoqing, Hou Xilin
State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, Nanjing 210095, China.
Anhui Jianghuai Horticulture Seeds Co., Ltd., Hefei 230000, China.
Plants (Basel). 2023 Oct 10;12(20):3523. doi: 10.3390/plants12203523.
A well-developed root system is crucial for the rapid growth, asexual reproduction, and adaptation to the drought environments of the watercress. After analyzing the transcriptome of the watercress root system, we found that a high concentration of auxin is key to its adaptation to dry conditions. For the first time, we obtained watercress, which revealed the dynamic distribution of auxin in watercress root development under drought conditions. Via the application of naphthylphthalamic acid (NPA), 4-biphenylboronic acid (BBO), ethylene (ETH), abscisic acid (ABA), and other factors, we confirmed that auxin has a significant impact on the root development of watercress. Finally, we verified the role of auxin in root development using watercress and showed that the synthesis of auxin in the root system mainly depends on the tryptophan, phenylalanine, and tyrosine amino acids (TAA) synthesis pathway. After the level of auxin increases, the root system of the watercress develops toward adaptation to dry environments. The formation of root aerenchyma disrupts the concentration gradient of auxin and is a key factor in the differentiation of lateral root primordia and H cells in watercress.
发育良好的根系对于豆瓣菜的快速生长、无性繁殖以及适应干旱环境至关重要。在分析了豆瓣菜根系的转录组后，我们发现高浓度的生长素是其适应干旱条件的关键。我们首次获得了揭示干旱条件下豆瓣菜根系发育过程中生长素动态分布的结果。通过施用萘基邻苯二甲酸（NPA）、4-联苯硼酸（BBO）、乙烯（ETH）、脱落酸（ABA）等因素，我们证实生长素对豆瓣菜根系发育有显著影响。最后，我们利用豆瓣菜验证了生长素在根系发育中的作用，并表明根系中生长素的合成主要依赖于色氨酸、苯丙氨酸和酪氨酸氨基酸（TAA）合成途径。生长素水平升高后，豆瓣菜的根系朝着适应干旱环境的方向发育。根通气组织的形成破坏了生长素的浓度梯度，是豆瓣菜侧根原基和H细胞分化的关键因素。
