Ramezani Sanaz, Javadi Iraj, Kokhdan Esmaeel Panahi, Omidifar Navid, Nikbakht Jafar, Sadeghi Heibatollah, Doustimotlagh Amir Hossein, Danaei Nazanin, Abbasi Reza, Sadeghi Hossein
Department of Toxicology, Shahreza Branch, Islamic Azad University, Shahreza, I.R. Iran.
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, I.R. Iran.
Res Pharm Sci. 2020 Dec 30;16(1):94-102. doi: 10.4103/1735-5362.305192. eCollection 2021 Feb.
Pulmonary fibrosis is a chronic disease of the lungs caused by inflammation, species of reactive oxygen, and immune defects. Antioxidant properties of has been reported in some studies. Therefore, the objective of the current study was to evaluate the effect of ethanolic extract of (EENO) on bleomycin (BLM)-induced lung fibrosis in rats.
Forty adult male Wistar rats (180-220 g) were randomly divided into 5 experimental groups. Normal control, BLM control received a single dose of BLM (6 IU/kg) intratracheally only on the first day, EENO + BLM group received EENO (500 mg/kg) one week before intratracheal BLM instillation and two weeks afterward, BLM + EENO group and BML + vitamin E group received EENO (500 mg/kg) and vitamin E (500 mg/kg) half-hour after BLM installation, respectively. The animals were sacrificed on day 22. Change in body weight, lung index, serum level of malondialdehyde (MDA) and nitric oxide (NO) metabolite, lung tissue hydroxyproline content and lung pathology were assessed.
FINDINGS/RESULTS: Pre- or post-treatment with EENO attenuated pulmonary fibrosis as evidenced by normalized lung index, improved histological changes and inhibited collagen deposition (hydroxyproline) in the animal lung. EENO also decreased MDA and NO metabolite release in comparison to the BLM control. vitamin E (500 mg/ kg) also significantly inhibited the BLM-induced lung toxicity.
EENO can prevent BLM-induced lung fibrosis in rats antioxidant activities. However, more studies are needed to elicit the exact mechanism of this effect.
肺纤维化是一种由炎症、活性氧种类和免疫缺陷引起的慢性肺部疾病。一些研究报道了[具体物质]的抗氧化特性。因此，本研究的目的是评估[具体物质]乙醇提取物（EENO）对博来霉素（BLM）诱导的大鼠肺纤维化的影响。
40只成年雄性Wistar大鼠（180 - 220克）随机分为5个实验组。正常对照组、BLM对照组仅在第一天经气管内给予单剂量的BLM（6 IU/kg），EENO + BLM组在经气管内注入BLM前一周和之后两周给予EENO（500毫克/千克），BLM + EENO组和BML + 维生素E组分别在注入BLM半小时后给予EENO（500毫克/千克）和维生素E（500毫克/千克）。在第22天处死动物。评估体重变化、肺指数、血清丙二醛（MDA）和一氧化氮（NO）代谢产物水平、肺组织羟脯氨酸含量以及肺病理学变化。
EENO预处理或后处理均可减轻肺纤维化，表现为肺指数正常化、组织学变化改善以及动物肺中胶原沉积（羟脯氨酸）受到抑制。与BLM对照组相比，EENO还降低了MDA和NO代谢产物的释放。维生素E（500毫克/千克）也显著抑制了BLM诱导的肺毒性。
EENO可通过抗氧化活性预防BLM诱导的大鼠肺纤维化。然而，需要更多研究来阐明这种作用的确切机制。
