Liu Hanghang, Chong Peifang, Yan Shipeng, Liu Zehua, Bao Xinguang, Tan Bingbing
College of Forestry, Gansu Agricultural University, Lanzhou 730070, China.
School of Forestry Engineering, Shandong Agriculture and Engineering University, Jinan 250100, China.
Plants (Basel). 2023 Oct 12;12(20):3542. doi: 10.3390/plants12203542.
This work aims at studying the molecular mechanisms underlying the response of to salt stress. We used RNA sequencing (RNA-Seq) and Tandem Mass Tag (TMT) techniques to identify differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in leaves treated with 0, 200, and 500 mM NaCl for 72 h. The results indicated that compared with the 0 mM NaCl treatment group, 2391 and 6400 DEGs were identified in the 200 and 500 mM NaCl treatment groups, respectively, while 47 and 177 DEPs were also identified. Transcriptome and proteome association analysis was further performed on leaves in the 0/500 mM NaCl treatment group, and 32 genes with consistent mRNA and protein expression trends were identified. , , , , , , and other differential genes are involved in photosynthesis, vesicle transport, auxin transport, and other functions of plants, and might play a key role in the salt tolerance of . In this study, transcriptome and proteome association techniques were used to screen candidate genes associated with salt tolerance in , which provides an important theoretical basis for understanding the molecular mechanism of salt tolerance in and breeding high-quality germplasm resources.
本研究旨在探究[植物名称]对盐胁迫响应的分子机制。我们运用RNA测序（RNA-Seq）和串联质谱标签（TMT）技术，鉴定了在0、200和500 mM NaCl处理72小时的[植物名称]叶片中的差异表达基因（DEGs）和差异表达蛋白（DEPs）。结果表明，与0 mM NaCl处理组相比，在200和500 mM NaCl处理组中分别鉴定出2391个和6400个DEGs，同时还鉴定出47个和177个DEPs。对0/500 mM NaCl处理组的[植物名称]叶片进一步进行转录组和蛋白质组关联分析，鉴定出32个mRNA和蛋白质表达趋势一致的基因。[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]、[基因名称5]、[基因名称6]和其他差异基因参与植物的光合作用、囊泡运输、生长素运输等功能，可能在[植物名称]的耐盐性中起关键作用。本研究利用转录组和蛋白质组关联技术筛选了与[植物名称]耐盐性相关的候选基因，为解析[植物名称]耐盐分子机制及培育优质种质资源提供了重要理论依据。
