Zhang Huilong, Liu Xiaowei, Yang Xiuyan, Wu Haiwen, Zhu Jianfeng, Zhang Huaxin
Research Center of Saline and Alkali Land of State Forestry and Grassland Administration, Beijing 100091, China.
Tianjin Research Institute of Forestry of Chinese Academy of Forestry, Tianjin 300450, China.
Plants (Basel). 2020 Mar 10;9(3):351. doi: 10.3390/plants9030351.
MicroRNAs (miRNAs) are endogenous small RNAs that play a crucial role in plant growth, development, and environmental stress responses. is a typical halophyte that is widely distributed in saline-alkali desert regions. Under salt stress, can complete germination, a critical biological process in the life cycle of seed plants. To identify miRNAs and predict target mRNAs involved in seed germination during salt stress, nine small-RNA libraries were constructed and analyzed from seeds treated with various concentrations of NaCl. We also obtained transcriptome data under the same treatment conditions. Further analysis identified 88 conserved miRNAs representing 25 defined families and discovered 13 novel miRNAs from nine libraries. A co-expression analysis was performed on the same samples to identify putative miRNA-mRNA interactions that were responsive to salt stress. A comparative analysis of expression during germination under 273 (threshold) and 43 mM (optimal) NaCl treatments identified 13 differentially expressed miRNAs and 23 corresponding target mRNAs, while a comparison between 43 mM NaCl and non-salt-stress conditions uncovered one differentially expressed miRNA and one corresponding target mRNA. These results provide basic data for further study of molecular mechanisms involved in the germination of salt-stressed seeds, and also provide a reference for the improvement of salt tolerance during plant germination.
微小RNA（miRNA）是一类内源性小RNA，在植物生长、发育及环境胁迫响应中发挥关键作用。[植物名称]是一种典型的盐生植物，广泛分布于盐碱荒漠地区。在盐胁迫下，[植物名称]能够完成种子植物生命周期中的一个关键生物学过程——萌发。为了鉴定参与盐胁迫下种子萌发的miRNA并预测其靶标mRNA，我们构建并分析了来自用不同浓度NaCl处理的[植物名称]种子的9个小RNA文库。我们还获得了相同处理条件下的转录组数据。进一步分析鉴定出代表25个已知家族的88个保守miRNA，并从9个文库中发现了13个新的miRNA。对相同样本进行共表达分析，以鉴定对盐胁迫有响应的假定miRNA-mRNA相互作用。在273 mM（阈值）和43 mM（最佳）NaCl处理下萌发过程中的表达比较分析鉴定出13个差异表达的miRNA和23个相应的靶标mRNA，而在43 mM NaCl与非盐胁迫条件之间的比较中发现了1个差异表达的miRNA和1个相应的靶标mRNA。这些结果为进一步研究盐胁迫下[植物名称]种子萌发所涉及的分子机制提供了基础数据，也为提高植物萌发期间的耐盐性提供了参考。
