Zhou Zhongjing, Liang Shuang, Zou Xiaowei, Teng Yi, Wang Weike, Fu Lizhong
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 311402, China.
Plants (Basel). 2023 Oct 13;12(20):3565. doi: 10.3390/plants12203565.
, a medicinal mushroom, has gained significant attention due to its beneficial properties. Phenolic acids are among the major bioactive compounds in , known for their antioxidant and anti-inflammatory activities. To precisely quantify the phenolic acid content, we developed a method utilizing ultra-high-performance liquid chromatography with triple quadrupole (UHPLC-QqQ). This study optimized the UHPLC-QqQ conditions to simultaneously separate and detect eight phenolic acids in (Pilát) L.W. Zhou and Y.C. Dai, including chlorogenic acid, -coumaric acid, caffeic acid, cryptochlorogenic acid, protocatechuic acid, ferulic acid, sinapic acid, and syringic acid. The separation process utilized a ZORBAX Eclipse Plus C18 column using 0.01% formic acid and 2 mmol/L ammonium formate in water as the aqueous phase and methanol containing 0.01% formic acid and 2 mmol/L ammonium formate as the organic phase. Calibration curves were constructed using standard solutions to quantitatively determine the phenolic acid content. The results showed significant variation in phenolic acid content among fruiting bodies, with Protocatechuic acid, -coumaric acid, and caffeic acid being the most abundant. This method is valuable for quantifying phenolic acid compounds under different cultivation conditions. It provides excellent sensitivity, selectivity, and reproducibility for the quantification of phenolic acids in , contributing to a better understanding of its chemical composition and potential health benefits. This approach represents a novel technical means for the simultaneous analysis of compound phenolic acids in fruiting bodies.
一种药用蘑菇，因其有益特性而备受关注。酚酸是该蘑菇中的主要生物活性化合物之一，以其抗氧化和抗炎活性而闻名。为了精确量化酚酸含量，我们开发了一种利用超高效液相色谱 - 三重四极杆（UHPLC - QqQ）的方法。本研究优化了UHPLC - QqQ条件，以同时分离和检测周氏和戴氏香菇（Pilát）L.W. Zhou和Y.C. Dai中的八种酚酸，包括绿原酸、对香豆酸、咖啡酸、隐绿原酸、原儿茶酸、阿魏酸、芥子酸和丁香酸。分离过程使用ZORBAX Eclipse Plus C18柱，以含0.01%甲酸和2 mmol/L甲酸铵的水作为水相，含0.01%甲酸和2 mmol/L甲酸铵的甲醇作为有机相。使用标准溶液构建校准曲线以定量测定酚酸含量。结果表明，香菇子实体中酚酸含量存在显著差异，原儿茶酸、对香豆酸和咖啡酸含量最为丰富。该方法对于量化不同栽培条件下的酚酸化合物具有重要价值。它为香菇中酚酸的定量提供了出色的灵敏度、选择性和重现性，有助于更好地了解其化学成分和潜在的健康益处。这种方法代表了一种同时分析香菇子实体中复合酚酸的新型技术手段。
