Wang Hao, Ma Jin-Xin, Wu Dong-Mei, Gao Neng, Si Jing, Cui Bao-Kai
Institute of Microbiology, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China.
Xinjiang Academy of Agricultural and Reclamation Sciences/Xinjiang Production and Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Biotechnology Research Institute, Shihezi 832000, China.
J Fungi (Basel). 2023 Feb 11;9(2):242. doi: 10.3390/jof9020242.
refers to a group of rare medicinal fungi with remarkable therapeutic properties. However, current knowledge on the bioactive ingredients and antioxidant activities of different species of this genus is limited. In this study, a total of 15 wild strains from 8 species of were selected as the experimental materials for identification of the bioactive components (polysaccharide, polyphenol, flavonoid, triterpenoid, and ascorbic acid) and antioxidant activities (scavenging activities against hydroxyl, superoxide, DPPH, and ABTS radicals; superoxide dismutase activity; and ferric reducing ability of plasma). Notably, individual strains contained different levels of various indicators, among which Cui 3573, Cui 14419 and Cui 14441, Dai 9061, and Dai 10841 displayed the strongest activities. The correlation analysis of bioactive ingredients and antioxidant activities revealed that the antioxidant capacity of is mainly associated with the contents of flavonoid and ascorbic acid, followed by polyphenol and triterpenoid, and finally, polysaccharide. Together, the results obtained from the comprehensive and systematic comparative analyses contribute further potential resources and critical guidance for the separation, purification, and further development and utilization of bioactive agents from wild species, as well as the optimization of their artificial cultivation conditions.
指的是一组具有显著治疗特性的珍稀药用真菌。然而，目前关于该属不同物种的生物活性成分和抗氧化活性的知识有限。在本研究中，从8个物种中总共挑选了15个野生菌株作为实验材料，用于鉴定生物活性成分（多糖、多酚、黄酮类化合物、三萜类化合物和抗坏血酸）和抗氧化活性（对羟基、超氧阴离子、DPPH和ABTS自由基的清除活性；超氧化物歧化酶活性；以及血浆铁还原能力）。值得注意的是，各个菌株含有不同水平的各种指标，其中Cui 3573、Cui 14419和Cui 14441、Dai 9061以及Dai 10841表现出最强的活性。生物活性成分与抗氧化活性的相关性分析表明，该属的抗氧化能力主要与黄酮类化合物和抗坏血酸的含量相关，其次是多酚和三萜类化合物，最后是多糖。总之，从全面系统的比较分析中获得的结果为从野生该属物种中分离、纯化和进一步开发利用生物活性剂以及优化其人工栽培条件提供了更多潜在资源和关键指导。
