Liu Sushuang, Xue Qi, Zhu Shuying, Liu Yanmin, Zou Huasong
School of Life Sciences and Health, Huzhou College, Huzhou 313000, China.
Plants (Basel). 2023 Oct 17;12(20):3600. doi: 10.3390/plants12203600.
The root architecture of a range of host plants is altered in response to infection. This work aimed to identify host genes involved in root development during infection. A deficient mutant of the type III secretion system regulator was created in GMI1000. The mutant was impaired in virulence but showed a similar suppressive effect as wild-type GMI1000 on tomato root development. Based on comparative transcriptome analysis, 209 genes were found that showed the same changed expression pattern in GMI1000 and mutant infected roots relative to uninoculated roots. Among them, the wall-associated receptor kinase was substantially downregulated in GMI1000 and mutant infected roots. Knockdown of led to a shorter primary root length and fewer lateral roots in tomato as well as in . The is a pivotal target suppressed by to shape the altered root development during infection.
一系列寄主植物的根系结构会因感染而发生改变。这项研究旨在鉴定感染过程中参与根系发育的寄主基因。在GMI1000中构建了III型分泌系统调节因子的缺陷突变体。该突变体的毒力受损，但对番茄根系发育的抑制作用与野生型GMI1000相似。基于比较转录组分析，发现209个基因在GMI1000和突变体感染的根中相对于未接种根呈现相同的表达变化模式。其中，壁相关受体激酶在GMI1000和突变体感染的根中显著下调。在番茄以及[此处原文缺失具体植物名称]中敲低[此处原文缺失具体基因名称]会导致主根长度缩短和侧根数量减少。[此处原文缺失具体基因名称]是[此处原文缺失具体病原菌名称]在感染期间塑造根系发育改变所抑制的关键靶点。
