Zhang Mi, Otsuki Kouharu, Takahashi Reo, Kikuchi Takashi, Zhou Di, Li Ning, Li Wei
Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan.
Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Plants (Basel). 2023 Oct 19;12(20):3620. doi: 10.3390/plants12203620.
Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has emerged as a powerful tool for the rapid identification of compounds within natural resources. Daphnane diterpenoids, a class of natural compounds predominantly found in plants belonging to the Thymelaeaceae and Euphorbiaceae families, have attracted much attention due to their remarkable anticancer and anti-HIV activities. In the present study, the presence of daphnane diterpenoids in , a plant belonging to the Thymelaeaceae family, was investigated by LC-MS/MS analysis. As a result, 21 daphnane diterpenoids (-) in the stems of were detected. Among these, six major compounds (, , , , , and ) were isolated and their structures were unequivocally identified through a comprehensive analysis of the MS and NMR data. For the minor compounds (-, , , , and ), their structures were elucidated by in-depth MS/MS fragmentation analysis. This study represents the first disclosure of structurally diverse daphnane diterpenoids in , significantly contributing to our understanding of bioactive diterpenoids in plants within the Thymelaeaceae family.
液相色谱-串联质谱联用（LC-MS/MS）已成为快速鉴定自然资源中化合物的强大工具。瑞香烷二萜类化合物是一类主要存在于瑞香科和大戟科植物中的天然化合物，因其显著的抗癌和抗HIV活性而备受关注。在本研究中，通过LC-MS/MS分析研究了瑞香科植物中瑞香烷二萜类化合物的存在情况。结果，在该植物的茎中检测到21种瑞香烷二萜类化合物（-）。其中，分离出六种主要化合物（、、、、和），并通过对质谱和核磁共振数据的综合分析明确鉴定了它们的结构。对于次要化合物（-、、、和），通过深入的MS/MS碎片分析阐明了它们的结构。本研究首次揭示了该植物中结构多样的瑞香烷二萜类化合物，对我们理解瑞香科植物中的生物活性二萜类化合物有重要贡献。
