Mannaa Mohamed, Han Gil, Jung Hyejung, Park Jungwook, Kim Jin-Cheol, Park Ae Ran, Seo Young-Su
Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
Department of Plant Pathology, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Plants (Basel). 2023 Oct 23;12(20):3653. doi: 10.3390/plants12203653.
The Korean fir tree , an endangered species in Korea, faces threats primarily from climate change-induced stress and drought. This study proposed a sustainable method to enhance drought tolerance using a black yeast-like fungus identified as (AK10). The 16S/ITS metabarcoding analysis assessed the impact of drought and AK10 treatment on the seedlings' rhizosphere microbiome. Results revealed a profound drought influence on the microbiome, particularly affecting fungal mycobiota. Drought-stressed seedlings exhibited elevated Agaricaceae levels, opportunistic fungi generally associated with decomposition. AK10 treatment significantly mitigated this proliferation and increased the relative abundance of beneficial fungi like and , known biocontrol agents and phosphate solubilizers. A notable reduction in the phytopathogenic levels was observed with AK10, alongside an increase in beneficial bacteria, including and . Furthermore, the conducted correlation analysis shed light on microbial interrelationships within the rhizosphere, elucidating potential co-associations and antagonisms. Taken together, the isolated AK10 identified in this study serves as a potential biostimulant, enhancing the drought tolerance in through beneficial alterations in the rhizosphere microbiome. This approach presents a promising strategy for the conservation of this endangered species.
朝鲜冷杉是韩国的濒危物种，主要面临气候变化引起的胁迫和干旱的威胁。本研究提出了一种可持续方法，利用一种被鉴定为（AK10）的类黑酵母真菌提高耐旱性。16S/ITS宏条形码分析评估了干旱和AK10处理对幼苗根际微生物群的影响。结果表明，干旱对微生物群有深远影响，尤其影响真菌菌群。受干旱胁迫的幼苗中伞菌科水平升高，伞菌科是通常与分解相关的机会性真菌。AK10处理显著减轻了这种增殖，并增加了有益真菌如 和 的相对丰度，这两种真菌是已知的生物防治剂和磷酸盐溶解剂。观察到AK10处理后植物病原菌 水平显著降低，同时有益细菌如 和 的数量增加。此外，进行的相关性分析揭示了根际内微生物的相互关系，阐明了潜在的共关联和拮抗作用。综上所述，本研究中分离出的AK10可作为一种潜在的生物刺激剂，通过根际微生物群的有益改变提高朝鲜冷杉的耐旱性。这种方法为保护这种濒危物种提供了一种有前景的策略。
