Mutreja Isha, Lan Caixia, Li Qishun, Aparicio Conrado
MDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics, Minneapolis, MN 55455, USA.
The Affiliated Stomatological Hospital of Nanchang University, Nanchang 330000, China.
Pharmaceutics. 2023 Oct 4;15(10):2418. doi: 10.3390/pharmaceutics15102418.
Dental implant-associated infection is a clinical challenge which poses a significant healthcare and socio-economic burden. To overcome this issue, developing antimicrobial surfaces, including antimicrobial peptide coatings, has gained great attention. Different physical and chemical routes have been used to obtain these biofunctional coatings, which in turn might have a direct influence on their bioactivity and functionality. In this study, we present a silane-based, fast, and efficient chemoselective conjugation of antimicrobial peptides (Cys-GL13K) to coat titanium implant surfaces. Comprehensive surface analysis was performed to confirm the surface functionalization of as-prepared and mechanically challenged coatings. The antibacterial potency of the evaluated surfaces was confirmed against both Streptococcus gordonii and Streptococcus mutans, the primary colonizers and pathogens of dental surfaces, as demonstrated by reduced bacteria viability. Additionally, human dental pulp stem cells demonstrated long-term viability when cultured on Cys-GL13K-grafted titanium surfaces. Cell functionality and antimicrobial capability against multi-species need to be studied further; however, our results confirmed that the proposed chemistry for chemoselective peptide anchoring is a valid alternative to traditional site-unspecific anchoring methods and offers opportunities to modify varying biomaterial surfaces to form potent bioactive coatings with multiple functionalities to prevent infection.
牙种植体相关感染是一项临床挑战，会带来重大的医疗和社会经济负担。为克服这一问题，开发抗菌表面，包括抗菌肽涂层，已受到广泛关注。人们采用了不同的物理和化学途径来获得这些生物功能涂层，而这些途径反过来可能会对其生物活性和功能产生直接影响。在本研究中，我们展示了一种基于硅烷的、快速且高效的抗菌肽（Cys-GL13K）化学选择性共轭方法，用于包覆钛种植体表面。进行了全面的表面分析，以确认制备好的涂层以及经机械挑战后的涂层的表面功能化。通过降低细菌活力证明，所评估表面对戈登链球菌和变形链球菌（牙表面的主要定植菌和病原体）均具有抗菌效力。此外，人牙髓干细胞在Cys-GL13K接枝的钛表面培养时表现出长期活力。细胞功能和对多种细菌的抗菌能力需要进一步研究；然而，我们的结果证实，所提出的化学选择性肽锚定方法是传统非特异性锚定方法的有效替代方案，并为修饰不同的生物材料表面提供了机会，以形成具有多种功能的强效生物活性涂层来预防感染。
