Bouassida Khaireddine, Marzouk Manel, Nouir Sahar, Ghammem Rim, Sahtout Wissal, Ghardallou Meriam, Fathallah Neila, Boukadida Jalel, Jaidane Mehdi, Slim Raoudha, Zaïri Amira
Urology Department, Sahloul Hospital, Sousse 4054, Tunisia.
Laboratory of Microbiology, Hospital Farhat Hached, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia.
Pharmaceutics. 2023 Oct 19;15(10):2496. doi: 10.3390/pharmaceutics15102496.
Ureteral double-J stents are frequently used to prevent urinary obstruction. They can develop bacterial colonization and encrustation, which leads to persistent infections that seldom respond to antibiotic treatment. Thus, the goal of this study was to evaluate the local spectrum of bacterial pathogens and their susceptibility to natural compounds. A total of 59 double-J ureteral stents from 59 consecutive patients were examined. The samples were inoculated on agar culture mediums. Extracts of L. were evaluated for their antibacterial activity with the diffusion and broth dilution methods; for antibiofilm activity, the crystal violet assay was used. The identification and the quantification of the different constituents of extracts were determined by reverse-phase high-performance liquid chromatography (RP-HPLC). Bacterial growth was found in three patients (5.1%). (1.7%), (1.7%), and (1.7%) strains were more commonly detected. They were resistant to several common antibiotics. All extracts presented several components, mainly nepetin-7-glucoside and trans-ferulic-acid, and they had antibacterial activity (MIC = 6.25 mg/mL and MBC = 6.25 mg/mL), and antibiofilm (59.70% at 25 mg/mL) properties, especially against . The results achieved confirm the important role of this plant as a source of therapeutic activities.
输尿管双J支架常用于预防尿路梗阻。它们会发生细菌定植和结壳，从而导致持续性感染，很少对抗生素治疗有反应。因此，本研究的目的是评估细菌病原体的局部谱及其对天然化合物的敏感性。对连续59例患者的59个双J输尿管支架进行了检查。将样本接种在琼脂培养基上。用扩散法和肉汤稀释法评估L.提取物的抗菌活性；对于抗生物膜活性，使用结晶紫测定法。通过反相高效液相色谱法（RP-HPLC）测定提取物不同成分的鉴定和定量。在3例患者（5.1%）中发现细菌生长。（1.7%）、（1.7%）和（1.7%）菌株更常被检测到。它们对几种常见抗生素耐药。所有提取物都含有几种成分，主要是荆芥苷-7-葡萄糖苷和反式阿魏酸，它们具有抗菌活性（MIC = 6.25 mg/mL，MBC = 6.25 mg/mL）和抗生物膜特性（25 mg/mL时为59.70%），尤其是对。所取得的结果证实了这种植物作为治疗活性来源的重要作用。
