Arthropod-Borne Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS 66502, USA.
Department of Entomology, Kansas State University, Manhattan, KS 66502, USA.
Viruses. 2023 Oct 18;15(10):2108. doi: 10.3390/v15102108.
Viruses that are transmitted by arthropods, or arboviruses, have evolved to successfully navigate both the invertebrate and vertebrate hosts, including their immune systems. Biting midges transmit several arboviruses including vesicular stomatitis virus (VSV). To study the interaction between VSV and midges, we characterized the transcriptomic responses of VSV-infected and mock-infected cells at 1, 8, 24, and 96 h post inoculation (HPI). The transcriptomic response of VSV-infected cells at 1 HPI was significant, but by 8 HPI there were no detectable differences between the transcriptome profiles of VSV-infected and mock-infected cells. Several genes involved in immunity were upregulated ( and ) or downregulated ( and ) in VSV-treated cells at 1 HPI. These results indicate that VSV infection in midge cells produces an early immune response that quickly wanes, giving insight into in vivo VSV tolerance that may underlie their permissiveness as vectors for this virus.
通过节肢动物传播的病毒，或虫媒病毒，已经进化到能够成功地在无脊椎动物和脊椎动物宿主中导航，包括它们的免疫系统。蠓传播几种虫媒病毒，包括水疱性口炎病毒（VSV）。为了研究 VSV 与蠓之间的相互作用，我们在接种后 1、8、24 和 96 小时（HPI）时对 VSV 感染和模拟感染的细胞的转录组反应进行了特征分析。1 HPI 时 VSV 感染细胞的转录组反应是显著的，但到 8 HPI 时，VSV 感染和模拟感染细胞的转录组图谱之间没有检测到差异。一些参与免疫的基因在 VSV 处理的细胞中上调（和）或下调（和）。这些结果表明，VSV 在蠓细胞中的感染会产生早期免疫反应，这种反应很快减弱，这为体内 VSV 耐受提供了线索，这种耐受可能是它们作为该病毒载体的许可基础。
