Tng Priscilla Y L, Al-Adwani Laila, Pauletto Egle, Hui Joshua Y K, Netherton Christopher L
The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UK.
The Institute of Medical Sciences, Foresterhill, University of Aberdeen, Aberdeen AB25 2ZD, UK.
Vaccines (Basel). 2023 Oct 10;11(10):1577. doi: 10.3390/vaccines11101577.
African swine fever (ASF) is a lethal disease in pigs that has grave socio-economic implications worldwide. For the development of vaccines against the African swine fever virus (ASFV), immunogenic antigens that generate protective immune responses need to be identified. There are over 150 viral proteins-many of which are uncharacterized-and humoral immunity to ASFV has not been closely examined. To profile antigen-specific antibody responses, we developed luciferase-linked antibody capture assays (LACAs) for a panel of ASFV capsid proteins and screened sera from inbred and outbred animals that were previously immunized with low-virulent ASFV before challenge with virulent ASFV. Antibodies to B646L/p72, D117L/p17, M1249L, and E120R/p14.5 were detected in this study; however, we were unable to detect B438L-specific antibodies. Anti-B646L/p72 and B602L antibodies were associated with recovery from disease after challenges with genotype I OUR T88/1 but not genotype II Georgia 2007/1. Antibody responses against M1249L and E120R/p14.5 were observed in animals with reduced clinical signs and viremia. Here, we present LACAs as a tool for the targeted profiling of antigen-specific antibody responses to inform vaccine development.
非洲猪瘟（ASF）是一种猪的致死性疾病，在全球范围内具有严重的社会经济影响。为了研发针对非洲猪瘟病毒（ASFV）的疫苗，需要鉴定能产生保护性免疫反应的免疫原性抗原。ASFV有150多种病毒蛋白，其中许多尚未被鉴定，并且针对ASFV的体液免疫尚未得到深入研究。为了分析抗原特异性抗体反应，我们针对一组ASFV衣壳蛋白开发了荧光素酶连接抗体捕获试验（LACA），并筛选了来自近交和远交动物的血清，这些动物在受到强毒ASFV攻击之前曾用低毒ASFV免疫过。在本研究中检测到了针对B646L/p72、D117L/p17、M1249L和E120R/p14.5的抗体；然而，我们未能检测到B438L特异性抗体。抗B646L/p72和B602L抗体与I型OUR T88/1攻击后疾病的恢复相关，但与II型格鲁吉亚2007/1攻击后无关。在临床症状和病毒血症减轻的动物中观察到了针对M1249L和E120R/p14.5的抗体反应。在此，我们将LACA作为一种工具，用于对抗原特异性抗体反应进行靶向分析，以为疫苗研发提供信息。
