Hsu Y L, Engelking H M, Leong J C
Appl Environ Microbiol. 1986 Dec;52(6):1353-61. doi: 10.1128/aem.52.6.1353-1361.1986.
The virion protein patterns of 71 isolates of infectious hematopoietic necrosis virus (IHNV) from the Pacific Northwest were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of [35S]-methionine-labeled virus. This analysis led to the classification of these virus isolates into four or more types. Type 1 virus was characterized by a nucleocapsid protein with an approximate molecular weight of 40,500. Type 2 and type 3 viruses have nucleocapsid proteins with molecular weights of 42,800 and 43,250, respectively. Type 2 virus was responsible for the recent epizootics of IHNV among fish in the lower Columbia River. The California IHNV isolates were type 3 with the exception of some of those isolated from fish at the Coleman Hatchery on the Sacramento River. These Coleman Hatchery isolates belonged to a type 4 virus group characterized by a larger glycoprotein of approximately 70,000 molecular weight. All other viruses examined had glycoproteins of 67,000 molecular weight. The "type 5" virus isolates were grouped together because they were not sufficiently distinct to warrant classification into a separate type. These findings have been useful in determining that a particular virus type is characteristic for a geographic area and will infect many different salmonid species in that area and the same type isolated from parental fish is responsible for the subsequent outbreak of the diseases in progeny.
通过对用[35S] - 甲硫氨酸标记的病毒进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳，分析了来自太平洋西北部的71株传染性造血坏死病毒（IHNV）的病毒粒子蛋白模式。该分析导致这些病毒分离株被分类为四种或更多种类型。1型病毒的特征是核衣壳蛋白的分子量约为40,500。2型和3型病毒的核衣壳蛋白分子量分别为42,800和43,250。2型病毒是最近哥伦比亚河下游鱼类中IHNV流行的原因。加利福尼亚州的IHNV分离株除了从萨克拉门托河科尔曼孵化场的鱼类中分离出的一些之外，均为3型。这些科尔曼孵化场分离株属于4型病毒组，其特征是具有约70,000分子量的较大糖蛋白。所有其他检测的病毒都有67,000分子量的糖蛋白。“5型”病毒分离株被归为一组，因为它们的差异不够明显，不足以被分类为一个单独的类型。这些发现有助于确定特定病毒类型是某个地理区域的特征，并且将感染该区域内许多不同的鲑科鱼类物种，并且从亲鱼分离出的相同类型病毒会导致后代随后爆发疾病。
