Dongying Key Laboratory of Salt Tolerance Mechanism and Application of Halophytes, Dongying Institute, Shandong Normal University, Dongying 257000, China; Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Science, Shandong Normal University, Jinan 250014, China.
Shandong Center for Disease Control and Prevention, Jinan 250013, China.
Int J Biol Macromol. 2024 Jan;254(Pt 1):127642. doi: 10.1016/j.ijbiomac.2023.127642. Epub 2023 Oct 28.
Overuse of insecticides has led to severe environmental problems. Insect cuticle, which consists mainly of chitin, proteins and a thin outer lipid layer, serves multiple functions. Its prominent role is as a physical barrier that impedes the penetration of xenobiotics, including insecticides. Blattella germanica (L.) is a major worldwide indoor pest that causes allergic disease and asthma. Extensive use of pyrethroid insecticides, including β-cypermethrin, has selected for the rapid and independent evolution of resistance in cockroach populations on a global scale. We demonstrated that BgCPLCP1, the first CPLCP (cuticular proteins of low complexity with a highly repetitive proline-rich region) family cuticular protein in order Blattodea, contributes to insecticide penetration resistance. Silencing BgCPLCP1 resulted in 85.0 %-85.7 % and 81.0 %-82.0 % thinner cuticle (and especially thinner endocuticle) in the insecticide-susceptible (S) and β-cypermethrin-resistant (R) strains, respectively. The thinner and more permeable cuticles resulted in 14.4 % and 20.0 % lower survival of β-cypermethrin-treated S- and R-strain cockroaches, respectively. This study advances our understanding of cuticular penetration resistance in insects and opens opportunities for the development of new efficiently and environmentally friendly insecticides targeting the CPLCP family of cuticular proteins.
过度使用杀虫剂导致了严重的环境问题。昆虫的外骨骼主要由几丁质、蛋白质和一层薄薄的外层脂质组成，具有多种功能。它的突出作用是作为一种物理屏障，阻止包括杀虫剂在内的外来物质的渗透。德国小蠊（L.）是一种全球性的室内害虫，会引起过敏疾病和哮喘。拟除虫菊酯杀虫剂的广泛使用，包括β-氯氰菊酯，已经在全球范围内选择了蟑螂种群对杀虫剂的快速和独立的抗性进化。我们证明，BgCPLCP1 是鞘翅目昆虫中第一个 CPLCP（低复杂度的甲壳蛋白，具有高度重复的脯氨酸丰富区）家族的甲壳蛋白，有助于杀虫剂穿透抗性。BgCPLCP1 的沉默导致对杀虫剂敏感（S）和β-氯氰菊酯抗性（R）菌株的外骨骼分别变薄 85.0%-85.7%和 81.0%-82.0%，特别是内骨骼更薄。更薄、更具渗透性的外骨骼导致β-氯氰菊酯处理的 S-和 R-菌株蟑螂的存活率分别降低了 14.4%和 20.0%。这项研究增进了我们对昆虫表皮穿透抗性的理解，并为开发针对 CPLCP 家族表皮蛋白的新型高效、环保的杀虫剂提供了机会。
