Faculty of Dentistry, The University of Hong Kong, Hong Kong, S.A.R, People's Republic of China.
University of Oklahoma College of Dentistry, Oklahoma, USA.
Sci Rep. 2023 Oct 29;13(1):18543. doi: 10.1038/s41598-023-45636-9.
We compare the effect of calcium and phosphate-containing sodium fluoride (NaF) varnishes to conventional NaF varnish on S. mutans and L. fermentum counts. 3-4 years old children were grouped according to their caries status (n = 45 each): caries-free, with non-cavitated and with cavitated lesions. Each group was randomly subdivided (n = 15 each) into: Group 1- 5% NaF, Group 2- 5% NaF with tricalcium phosphate, Group 3- 5% NaF with casein phosphopeptide- amorphous calcium phosphate. Biofilm and saliva were collected to quantify microorganisms at baseline (T1) and 24-months (T2). Differences between groups were compared using Kruskal-Wallis test, followed by Dunn-Bonferroni post-test, at 0.0167 α-level. Significant difference was found for percentage of children with detectable biofilm L. fermentum (p = 0.013) at T1 and salivary S. mutans (p = 0.011) at T2. Percentage of children increased from T1 to T2 in Group 2 with salivary S. mutans (p = 0.007), salivary L. fermentum (p = 0.035), and biofilm L. fermentum (p = 0.019) and in Group 3 with salivary L. fermentum (p = 0.035). Bacterial change was not significant in both samples of intervention groups, except increase in salivary S. mutans (p = 0.038) in Group 3. Both calcium- and phosphate-containing NaF varnishes demonstrated similar antibacterial effect on S. mutans and L. fermentum compared to conventional NaF varnish.
我们比较了含钙和磷酸盐的氟化物（NaF）涂料与常规 NaF 涂料对变形链球菌和发酵乳杆菌计数的影响。根据龋齿状况将 3-4 岁儿童分组（每组 45 人）：无龋、无龋窝沟和有龋窝沟。每组再随机分为（每组 15 人）：第 1 组-5%NaF、第 2 组-5%NaF 加磷酸三钙、第 3 组-5%NaF 加酪蛋白磷酸肽-无定形磷酸钙。基线（T1）和 24 个月（T2）时采集生物膜和唾液以定量微生物。使用 Kruskal-Wallis 检验比较组间差异，然后使用 Dunn-Bonferroni 事后检验，α 值为 0.0167。在 T1 时可检测到生物膜发酵乳杆菌的儿童百分比（p=0.013）和 T2 时唾液变形链球菌（p=0.011）存在显著差异。T2 时第 2 组唾液变形链球菌（p=0.007）、唾液发酵乳杆菌（p=0.035）和生物膜发酵乳杆菌（p=0.019）和第 3 组唾液发酵乳杆菌（p=0.035）儿童百分比增加。除第 3 组唾液变形链球菌（p=0.038）外，干预组两种样本的细菌变化均不显著。含钙和磷酸盐的 NaF 涂料与常规 NaF 涂料相比，对变形链球菌和发酵乳杆菌均具有相似的抗菌作用。
