Department of Pediatric Dentistry and Dental Public Health, Faculty of Dentistry, Alexandria University, Champollion St, Egypt, Azarita, Alexandria, 21527, Egypt.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Clin Oral Investig. 2024 Feb 23;28(3):167. doi: 10.1007/s00784-024-05562-0.
To compare the antibacterial effect of Nanosilver Fluoride varnish (NSF) varnish, P11-4 and Sodium Fluoride (NaF) varnish against salivary Streptococcus mutans (S. mutans) and Lactobacilli.
66 patients aged 10-24 years old were randomly assigned to receive single application of NSF, P11-4 or NaF varnish. Baseline unstimulated saliva samples were collected before the agents were applied and S.mutans and Lactobacilli colony forming units (CFU) were counted. After one, three and six months, microbiological samples were re-assessed. Groups were compared at each time point and changes across time were assessed. Multivariable linear regression compared the effect of P11-4 and NSF to NaF on salivary S. mutans and Lactobacilli log count at various follow up periods.
There was a significant difference in salivary S. mutans log count after 1 month between P11-4 (B= -1.29, p = 0.049) and NaF but not at other time points nor between NSF and NaF at any time point. The significant reduction in bacterial counts lasted up to one month in all groups, to three months after using P11-4 and NaF and returned to baseline values after six months.
In general, the antimicrobial effect of P11-4 and NSF on salivary S. mutans and Lactobacilli was not significantly different from NaF varnish. P11-4 induced greater reduction more quickly than the two other agents and NSF antibacterial effect was lost after one month.
NSF varnish and P11-4 have antimicrobial activity that does not significantly differ from NaF by 3 months. P11-4 has the greatest antibacterial effect after one month with sustained effect till 3 months. The antibacterial effect of NSF lasts for one month. NaF remains effective till 3 months.
This trial was prospectively registered on the clinicaltrials.gov registry with ID: NCT04929509 on 18/6/2021.
比较纳米氟化银涂料（NSF）涂料、P11-4 和氟化钠（NaF）涂料对唾液变形链球菌（S. mutans）和乳酸杆菌的抗菌效果。
将 66 名年龄在 10-24 岁的患者随机分为 NSF、P11-4 或 NaF 涂料单次应用组。在药物应用前采集基础非刺激唾液样本，计数 S. mutans 和乳酸杆菌的菌落形成单位（CFU）。1、3 和 6 个月后，重新评估微生物样本。在每个时间点比较各组，评估随时间的变化。多变量线性回归比较 P11-4 和 NSF 对 NaF 在不同随访期唾液 S. mutans 和 Lactobacilli 对数计数的影响。
在 1 个月时，P11-4（B=-1.29，p=0.049）和 NaF 组之间唾液 S. mutans 对数计数有显著差异，但在其他时间点或 NSF 和 NaF 之间无显著差异。在所有组中，细菌计数的显著减少持续到 1 个月，使用 P11-4 和 NaF 后持续到 3 个月，然后在 6 个月后恢复到基线值。
一般来说，P11-4 和 NSF 对唾液 S. mutans 和乳酸杆菌的抗菌效果与 NaF 涂料无显著差异。P11-4 诱导的减少作用比其他两种药物更快，且 NSF 的抗菌作用在 1 个月后丧失。
NSF 涂料和 P11-4 具有与 3 个月时的 NaF 相似的抗菌活性。P11-4 在 1 个月时有最大的抗菌效果，持续到 3 个月。NSF 的抗菌效果持续 1 个月。NaF 持续有效至 3 个月。
本试验于 2021 年 6 月 18 日在 clinicaltrials.gov 注册，ID：NCT04929509。
