Park Jaebum, Sathuvalli Vidyasagar, Yilma Solomon, Whitworth Jonathan, Novy Richard G
Small Grains and Potato Germplasm Research Station, United States Department of Agriculture - Agricultural Research Service, Aberdeen, ID, United States.
Hermiston Agricultural Research and Extension Center, Oregon State University, Hermiston, OR, United States.
Front Plant Sci. 2023 Oct 11;14:1222596. doi: 10.3389/fpls.2023.1222596. eCollection 2023.
Potato late blight (causal agent ) is a disease of potatoes with economic importance worldwide. Control is primarily through field monitoring and the application of fungicides. Control of late blight with fungicides and host plant resistance is difficult, with documented cases of such control measures failing with the advent of new pathotypes of . To better understand host plant resistance and to develop more durable late blight resistance, Quantitative Trait Locus/Loci (QTL) analysis was conducted on a tetraploid mapping population derived from late blight-resistant potato cultivar Palisade Russet. Additionally, QTL analyses for other traits such as Verticillium wilt and early blight resistance, vine size and maturity were performed to identify a potential relationship between multiple traits and prepare genetic resources for molecular markers useful in breeding programs. For this, one hundred ninety progenies from intercrossing Palisade Russet with a late blight susceptible breeding clone (ND028673B-2Russ) were assessed. Two parents and progenies were evaluated over a two-year period for response to infection by the US-8 genotype of in inoculated field screenings in Corvallis, Oregon. In Aberdeen, Idaho, the same mapping population was also evaluated for phenotypic response to early blight and Verticillium wilt, and vine size and maturity in a field over a two-year period. After conducting QTL analyses with those collected phenotype data, it was observed that chromosome 5 has a significant QTL for all five traits. Verticillium wilt and vine maturity QTL were also observed on chromosome 1, and vine size QTL was also found on chromosomes 3 and 10. An early blight QTL was also detected on chromosome 2. The QTL identified in this study have the potential for converting into breeder-friendly molecular markers for marker-assisted selection.
马铃薯晚疫病（致病因子）是一种在全球具有经济重要性的马铃薯病害。防治主要通过田间监测和施用杀菌剂来进行。利用杀菌剂和寄主植物抗性来控制晚疫病很困难，有记录表明随着新致病型的出现，此类控制措施会失效。为了更好地理解寄主植物抗性并培育更持久的晚疫病抗性，对源自晚疫病抗性马铃薯品种“栅栏赤褐”的四倍体作图群体进行了数量性状基因座（QTL）分析。此外，还对黄萎病和早疫病抗性、藤蔓大小和成熟度等其他性状进行了QTL分析，以确定多个性状之间的潜在关系，并为育种计划中有用的分子标记准备遗传资源。为此，评估了将“栅栏赤褐”与晚疫病易感育种克隆（ND028673B - 2Russ）杂交产生的190个后代。在俄勒冈州科瓦利斯的接种田间筛选中，对两个亲本和后代进行了为期两年的美国8型致病因子感染反应评估。在爱达荷州阿伯丁，同一作图群体也在田间进行了为期两年的早疫病、黄萎病表型反应以及藤蔓大小和成熟度评估。在用收集到的表型数据进行QTL分析后，发现第5号染色体对所有五个性状都有一个显著的QTL。在第1号染色体上也观察到了黄萎病和藤蔓成熟度QTL，在第3号和第10号染色体上也发现了藤蔓大小QTL。在第2号染色体上也检测到了一个早疫病QTL。本研究中鉴定出的QTL有可能转化为对育种者友好的分子标记用于标记辅助选择。
